|Application ||WB, IHC-F|
|Calculated MW||50985 Da|
|Purification||Antisera to tryptophan hydroxylase were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Recombinant rabbit Tryptophan Hydroxylase purified from E. coli.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Tryptophan 5-hydroxylase 1, 220.127.116.11, Tryptophan 5-monooxygenase 1, TPH1, TPH, TPRH, TRPH|
|Target/Specificity||ABD11496 recognises Tryptophan Hydroxylase (TPH) which catalyses the 5-hydroxylation of tryptophan, in the first stage of indoleamine biosynthesis. Serotonin biosynthesis occurs principally in neurons which originate in the raphe nucleus of the brain, and melatonin synthesis takes place within the pineal gland.|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Tryptophan Hydroxylase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Haycock, J. W. et al. (2002) A monoclonal antibody to tryptophan hydroxylase: applications and identification of the epitope.J. Neurosci. Methods 114: 205-212.
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