|Application ||WB, E, Func|
|Calculated MW||26790 Da|
|Purification||Antisera to chicken Interleukin 6 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.|
|Immunogen||Recombinant chicken IL-6(PAP003)|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interleukin-6, ChIL-6, IL-6, IL6|
|Target/Specificity||ABD11503 recognizes chicken Interleukin 6 (chIL-6).Mammalian IL-6 is a multifunctional pro-inflammatory cytokine, which plays a major role in the regulation of immune responses, haematopoiesis and the induction of acute phase responses. The action of IL-6 occurs via the signal transducing receptor chain gp130 and the IL-6 receptor alpha chain is responsible for ligand binding. IL-6 is produced by a wide variety of cells including B lymphocytes, T lymphocytes, monocytes/macrophages, fibroblasts and endothelial cells and has a widespread cellular effect, including B and T lymphocytes, hepatocytes and cells of the CNS. Studies relating to the properties of chIL-6 have been limited, but demonstrate that hepatocyte-stimulating factor (HSF), secreted by lipopolysaccharide (LPS)-stimulated macrophages, induces fibronectin production in cultured chicken hepatocytes, and that HSF produced by LPS-treated fibroblasts, was neutralized by an antiserum to recombinant HuIL-6 (rHuIL-6). rHuIL-6 has also been shown as biologically active on chicken hepatocytes. ABD11503 neutralizes chicken IL-6 in 7D1 bioassays.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Store at -20℃ only.|
|Precautions||Anti-Chicken Interleukin-6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
5. De Boever, S. et al. (2010) Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression.Avian Pathol. 39: 41-6.1. Staeheli, P. et al. (2001) Cytokines of Birds: Conserved functions – A largely different look.J. Interferon and Cytokine Research 21: 993-1010.2. Schneider, K. et al. (2001) Chicken interleukin-6. cDNA structure and biological properties.Eur. J. Biochem. 268: 4200-4206.3. Amrani, D.L. et al. (1986) Effect of hepatocyte-stimulating factor and glucocorticoids on plasma fibronectin levels.Biochem. J. 238(2): 365-371.4. Samad, F. et al. (1993) Stimulation of chick hepatocyte fibronectin production by fibroblast-conditioned medium is due to interleukin 6.Biochim. Biophys. Acta. 1181(3): 207-213.
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