|Application ||WB, E, IP, Func|
|Calculated MW||22116 Da|
|Purification||Antisera to chicken Interferon alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.|
|Immunogen||Recombinant chicken IFNα(PAP004)|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interferon type A1/A2, IFNA1|
|Target/Specificity||ABD11504 recognizes chicken Interferon alpha (chIFNα). The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent. ABD11504 cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma. In bioassays, this antiserum neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities, see Schwarz, H. et al. for details.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Store at -20℃ only.|
|Precautions||Anti-Chicken Interferon alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Schwarz, H. et al. (2004) Synthesis of IFN-beta by virus-infected chicken embryo cells demonstrated with specific antisera and a new bioassay.J. Interferon Cytokine Res. 24: 179-184.1. Staeheli, P. et al. (2001) Cytokines of Birds: Conserved functions – A largely different look.J. Interferon Cytokine Res. 21: 993-1010.2. Sick, C. et al. (1996) A family of genes coding for two serologically distinct chicken interferons.J. Biol. Chem. 271(13): 7635-7639. .3. Schultz, U. et al. (1995) Recombinant chicken interferon: a potent antiviral agent that lacks intrinsic macrophage activating factor activity.Eur. J. Immunol. 25: 847-851.
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