|Application ||WB, E, IP, Func|
|Calculated MW||22116 Da|
|Purification||Antisera to chicken Interferon alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.|
|Immunogen||Recombinant chicken IFNα(PAP004)|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interferon type A1/A2, IFNA1|
|Target/Specificity||ABD11504 recognizes chicken Interferon alpha (chIFNα). The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent. ABD11504 cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma. In bioassays, this antiserum neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities, see Schwarz, H. et al. for details.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Store at -20℃ only.|
|Precautions||Anti-Chicken Interferon alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Schwarz, H. et al. (2004) Synthesis of IFN-beta by virus-infected chicken embryo cells demonstrated with specific antisera and a new bioassay.J. Interferon Cytokine Res. 24: 179-184.1. Staeheli, P. et al. (2001) Cytokines of Birds: Conserved functions – A largely different look.J. Interferon Cytokine Res. 21: 993-1010.2. Sick, C. et al. (1996) A family of genes coding for two serologically distinct chicken interferons.J. Biol. Chem. 271(13): 7635-7639. .3. Schultz, U. et al. (1995) Recombinant chicken interferon: a potent antiviral agent that lacks intrinsic macrophage activating factor activity.Eur. J. Immunol. 25: 847-851.
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