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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Chicken Interferon alpha Antibody    

Anti-Chicken Interferon alpha Antibody

Rabbit Anti-Chicken Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E, IP, Func
Primary Accession P42165
Reactivity Chicken
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 22116 Da
Additional Information
Purification Antisera to chicken Interferon alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.
Immunogen Recombinant chicken IFNα(PAP004)
Shelf Life 18 months from date of despatch.
Gene ID 100857744;100857947;100858177;100859032;396398
Other Names Interferon type A1/A2, IFNA1
Target/Specificity ABD11504 recognizes chicken Interferon alpha (chIFNα). The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent. ABD11504 cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma. In bioassays, this antiserum neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities, see Schwarz, H. et al. for details.
Preservative & Stabilisers Preservative Free
Storage Store at -20℃ only.
PrecautionsAnti-Chicken Interferon alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Schwarz, H. et al. (2004) Synthesis of IFN-beta by virus-infected chicken embryo cells demonstrated with specific antisera and a new bioassay.
J. Interferon Cytokine Res. 24: 179-184.1. Staeheli, P. et al. (2001) Cytokines of Birds: Conserved functions – A largely different look.
J. Interferon Cytokine Res. 21: 993-1010.2. Sick, C. et al. (1996) A family of genes coding for two serologically distinct chicken interferons.
J. Biol. Chem. 271(13): 7635-7639. .3. Schultz, U. et al. (1995) Recombinant chicken interferon: a potent antiviral agent that lacks intrinsic macrophage activating factor activity.
Eur. J. Immunol. 25: 847-851.

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Cat# ABD11504
(40 western blots)
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