|Application ||WB, IHC-F, FC, IP|
|Calculated MW||30959 Da|
|Purification||Antisera to human LOX-1 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Recombinant human extracellular domain LOX-1 protein from bacteria.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Oxidized low-density lipoprotein receptor 1, Ox-LDL receptor 1, C-type lectin domain family 8 member A, Lectin-like oxidized LDL receptor 1, LOX-1, Lectin-like oxLDL receptor 1, hLOX-1, Lectin-type oxidized LDL receptor 1, Oxidized low-density lipoprotein receptor 1, soluble form, OLR1, CLEC8A, LOX1|
|Target/Specificity||Sheep anti-LOX-1 antibody recognizes the type II membrane glycoprotein LOX-1 (lectin-like oxidized LDL receptor-1), a 40-50kDa class E scavenger receptor, expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells. LOX-1 binds to a wide range of ligands, including oxidized low-density lipoprotein (oxLDL), hypochlorite modified high-density lipoprotein (HDL), aged/apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions.ABD11507 will detect LOX-1 on transfected cells and in recombinant protein reconstitution assays. LOX-1 levels are low in normal resting human tissues so may fall below detectable levels. Expression of the LOX-1 gene is upregulated by oxLDL and the binding of LOX-1 to oxLDL results in the activation of NF-kappaB. Furthermore, LOX-1 antibodies have been shown to suppress the oxidized HDL (oxHDL) activation of NF-KappaB in endothelial cells, suggesting that this activation may be due to the binding of oxHDL to LOX-1. Angiotensin II and the inflammatory cytokine Tumour Necrosis Factor alpha (TNFalpha) also invoke an increase in LOX-1 gene expression and studies have focused on its role in endothelial dysfunction (Chen, M. et al.)(Sawamura, T. et al.) and inflammatory diseases such as atherosclerosis (Murphy, J.E. et al.) and rheumatoid arthritis, as well as its affect on CD40/CD40L signaling in both atherosclerosis and human coronary artery endothelial cells (HCAECs) (Li, D. et al.).|
|Preservative & Stabilisers||0.09% Sodium Azide; 1% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human LOX-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Murphy, J.E. et al. (2006) LOX-1 scavenger receptor mediates calcium-dependent recognition of phosphatidylserine and apoptotic cells.Biochem J. 393: 107-15.1. Chen, M. et al. (2002) LOX-1, the receptor for oxidized low-density lipoprotein identified from endothelial cells: implications in endothelial dysfunction and atherosclerosis.Pharmacol. Ther. 95: 89-100. 2. Li, D. et al. (2003) LOX-1, an oxidized LDL endothelial receptor, induces CD40/CD40L signaling in human coronary artery endothelial cells.Atherosclerosis, Thrombosis and Vascular Biology 23: 816-821. 3. Morawietz, H. et al. (1999) Angiotensin II induces LOX-1, the human endothelial receptor for oxidized low-density lipoprotein.Circulation 100: 899-902. 4. Sawamura, T. et al. (1997) An endothelial receptor for oxidized low-density lipoprotein.Nature 386: 73-77. 5. Yoshida, H. et al. (1998) Identification of the lectin-like receptor for oxidized low-density lipoprotein in human macrophages and its potential role as a scavenger receptor.Biochem. J. 334: 9-13.
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