|Application ||WB, IF, E, IP, CHIP|
|Calculated MW||11367 Da|
|Purification||Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.|
|Immunogen||Ovalbumin-conjugated peptide: NSGRGAcKGGKGLCc|
|Shelf Life||18 months from date of despatch.|
|Other Names||Histone H4, HIST1H4A, H4/A, H4FA|
|Target/Specificity||ABD11511 is specific for Histone H4 acetylated at lysine 5.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Histone H4 (Ac5) Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Turner, B.M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabeling with site-specific antibodies.FEBS Lett. 253 (1-2): 141-5. 2. Turner, B.M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.Cell. 69 (2): 375-84. 3. Belyaev, N. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.Hum Genet. 97 (5): 573-8. 4. Auger, A. et al. (2008) Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variants.Mol Cell Biol. 28: 2257-70. 5. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.J Inflamm (Lond). 8 (1): 1. 6. Ito, K. et al. (2000) Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1beta-induced histone H4 acetylation on lysines 8 and 12.Mol Cell Biol. 20: 6891-903. 7. Nie, M. et al. (2003) Transcriptional regulation of cyclooxygenase 2 by bradykinin and interleukin-1beta in human airway smooth muscle cells: involvement of different promoter elements, transcription factors, and histone h4 acetylation.Mol Cell Biol. 23: 9233-44. 8. Nie, M. et al. (2005) beta2-Adrenoceptor agonists, like glucocorticoids, repress eotaxin gene transcription by selective inhibition of histone H4 acetylation.J Immunol. 175: 478-86. 9. Tanny, J.C. et al. (2004) Budding yeast silencing complexes and regulation of Sir2 activity by protein-protein interactions.Mol Cell Biol. 24: 6931-46. 10. Spada, F. et al. (2005) Histone H4 post-translational modifications in chordate mitotic and endoreduplicative cell cycles.J Cell Biochem. 95: 885-901. 11. Lacoste, N. et al. (2002) Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferase.J Biol Chem. 277: 30421-4. 12. McManus, K.J. et al. (2006) The transcriptional regulator CBP has defined spatial associations within interphase nuclei.PLoS Comput Biol. 2: e139. 13. Krogan, N.T. et al. (2012) APETALA2 negatively regulates multiple floral organ identity genes in Arabidopsis by recruiting the co-repressor TOPLESS and the histone deacetylase HDA19.Development. 139: 4180-90. 14. Havasi, A. et al. (2013) Histone Acetyl Transferase (HAT) HBO1 and JADE1 in Epithelial Cell Regeneration.Am J Pathol. 182: 152-62. 15. Miyamoto, Y. et al. (2015) Involvement of the Tyro3 receptor and its intracellular partner Fyn signaling in Schwann cell myelination.Mol Biol Cell. 26 (19): 3489-503.
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