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>   home   >   Products   >   Primary Antibodies   >   Anti-Histone H4 (Ac5) Antibody (Serum)   

Anti-Histone H4 (Ac5) Antibody (Serum)

     
  • WB - Anti-Histone H4 (Ac5) Antibody (Serum) ABD11511
    Published customer image:Rabbit anti Histone H4 antibody used for the detection of acetylated histone H4 in rat colitis tissue lysates by western blotting.Image caption:Acetylation on histone 4 specific lysine residues 5 (A), 8 (B), 12 (C) and 16 (D) in a Sham (control) and trinitrobenzene sulfonic acid (TNBS) rat model of colitis. Results were obtained by Western blotting. In order to evaluate changes in density from different western blotting experiments control densitometry was denoted as 100% and differences were accounted as increase percentage of the control. Representative examples of bands obtained are also illustrated. Columns represent the densitometric evaluation of three independent experiments (mean ± SEM). (*p < 0.05 vs Sham proximal or Sham distal respectively).From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N. Differential patterns of histone acetylation in inflammatory bowel diseases.J Inflamm (Lond). 2011 Jan 27;8:1.
  • IF - Anti-Histone H4 (Ac5) Antibody (Serum) ABD11511
    Published customer image:Rabbit anti Histone H4 antibody used for the detection of acetylated histone H5 in 10T1/2 cells by immunofluorescence.Image caption:Spatial Relationship between CBP and Specific Histone H3 Post-Translational Modifications The spatial relationship between CBP and a known product of its HAT activity, acetylated K5 (A), or an unrelated histone H3 modification, trimethylated K4 (B), was investigated. Representative and deconvolved high-resolution images (100×) of interphase 10T1/2 cells immunofluorescently labeled with anti-CBP and either anti-AcK9 or anti-tMeK4 counterstained with DAPI are presented. The merged image presents a single plane from the 3-D projection where CBP and AcK9 or tMeK4 are shown in green and red, respectively. A region, identified by the white box, is further magnified to show the spatial relationship between CBP and either post-translational histone modification. Scale bars represent 3 &mu;m.From: McManus KJ, Stephens DA, Adams NM, Islam SA, Freemont PS, Hendzel MJ The Transcriptional Regulator CBP Has Defined Spatial Associations within Interphase Nuclei.PLoS Comput Biol 2: e139.
  • WB - Anti-Histone H4 (Ac5) Antibody (Serum) ABD11511
    Published customer image:Rabbit anti Histone H4 antibody used for the detection of acetylated histone H4 in Dextran sulphate treated rat tissue lysates by western blotting.Image caption:Acetylation on histone 4 specific lysine residues 5 , 8 , 12 and 16 in Lewis and Sprague-Dawley dextran sulfate sodium (5% DSS). A: Representative bands of H4K5, K8, K12 and K16 acetylation. Lanes for Lewis rats represent: non-DSS treated and DSS-treated. Likewise representative bands are illustrated for the Sprague-Dawley rats. Graphical representation of Western blotting data. H4 acetylation of K5 (B), K8 (C), K12 (D) and K16 (E). Columns represent the mean ± SEM of three independent experiments.From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N. Differential patterns of histone acetylation in inflammatory bowel diseases.J Inflamm . 2011 Jan 27;8(1):1.
  • WB - Anti-Histone H4 (Ac5) Antibody (Serum) ABD11511
    Published customer image:Rabbit anti Histone H4 antibody used for the detection of acetylated histone H4 in intestinal tissue lysates by western blotting.Image caption:Acetylation on histone 4 and H4 lysine residues in Crohn&#39;s disease. Columns represent the mean ± SEM of three independent experiments. Four biopsies were pooled to obtain sufficient protein for one experiment (50 µg of protein) (*p < 0.05 vs control). Pan acetylation on H4 in Crohn&#39;s disease (A). Acetylation on histone 4 specific lysine residues 5 (B), K8 (C), K12 (D), and 16 (E), in non-inflamed, inflamed tissue and Peyer&#39;s patches of Crohn&#39;s disease patients. Results were obtained by Western blotting. Columns represent the mean ± SEM of three independent experiments. (*p < 0.05 vs control, #p < 0.005 vs non-inflamed CD). Representative images of the bands obtained are illustrated.From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N. Differential patterns of histone acetylation in inflammatory bowel diseases.J Inflamm . 2011 Jan 27;8(1):1.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E, IP, CHIP
Primary Accession P62805
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 11367 Da
Additional Information
Other Species Mammalian,D,Amphibia,Y,Plants
Purification Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.
Immunogen Ovalbumin-conjugated peptide: NSGRGAcKGGKGLCc
Shelf Life 18 months from date of despatch.
Gene ID 121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
Other Names Histone H4, HIST1H4A, H4/A, H4FA
Target/Specificity ABD11511 is specific for Histone H4 acetylated at lysine 5.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Histone H4 (Ac5) Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. Turner, B.M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabeling with site-specific antibodies.
FEBS Lett. 253 (1-2): 141-5. 2. Turner, B.M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.
Cell. 69 (2): 375-84. 3. Belyaev, N. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.
Hum Genet. 97 (5): 573-8. 4. Auger, A. et al. (2008) Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variants.
Mol Cell Biol. 28: 2257-70. 5. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 8 (1): 1. 6. Ito, K. et al. (2000) Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1beta-induced histone H4 acetylation on lysines 8 and 12.
Mol Cell Biol. 20: 6891-903. 7. Nie, M. et al. (2003) Transcriptional regulation of cyclooxygenase 2 by bradykinin and interleukin-1beta in human airway smooth muscle cells: involvement of different promoter elements, transcription factors, and histone h4 acetylation.
Mol Cell Biol. 23: 9233-44. 8. Nie, M. et al. (2005) beta2-Adrenoceptor agonists, like glucocorticoids, repress eotaxin gene transcription by selective inhibition of histone H4 acetylation.
J Immunol. 175: 478-86. 9. Tanny, J.C. et al. (2004) Budding yeast silencing complexes and regulation of Sir2 activity by protein-protein interactions.
Mol Cell Biol. 24: 6931-46. 10. Spada, F. et al. (2005) Histone H4 post-translational modifications in chordate mitotic and endoreduplicative cell cycles.
J Cell Biochem. 95: 885-901. 11. Lacoste, N. et al. (2002) Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferase.
J Biol Chem. 277: 30421-4. 12. McManus, K.J. et al. (2006) The transcriptional regulator CBP has defined spatial associations within interphase nuclei.
PLoS Comput Biol. 2: e139. 13. Krogan, N.T. et al. (2012) APETALA2 negatively regulates multiple floral organ identity genes in Arabidopsis by recruiting the co-repressor TOPLESS and the histone deacetylase HDA19.
Development. 139: 4180-90. 14. Havasi, A. et al. (2013) Histone Acetyl Transferase (HAT) HBO1 and JADE1 in Epithelial Cell Regeneration.
Am J Pathol. 182: 152-62. 15. Miyamoto, Y. et al. (2015) Involvement of the Tyro3 receptor and its intracellular partner Fyn signaling in Schwann cell myelination.
Mol Biol Cell. 26 (19): 3489-503.

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