|Calculated MW||49736 Da|
|Purification||Purified IgG was prepared by affinity chromatography.|
|Immunogen||A 14 amino acid peptide from the centre of human Caspase-5.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Caspase-5, CASP-5, 188.8.131.52, ICE(rel)-III, Protease ICH-3, Protease TY, Caspase-5 subunit p20, Caspase-5 subunit p10, CASP5, ICH3|
|Target/Specificity||Rabbit anti-Human Caspase-5 antibody recognizes an epitope located within the internal region of human Caspase-5, a 47kDa cysteine protease, otherwise known as ICH-3. Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes.Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta.Thein vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma (IFNg). In vitro studies have shown cleavage of Caspase-5 by the serine protease Granzyme B, which plays a major role in Cytotoxic T lymphocyte (CTL)-induced apoptosis (Wanget al.1996).|
|Preservative & Stabilisers||0.02% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Caspase-5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Wang, S. et al. (1996) Identification and characterization of Ich-3, a member of the Interleukin-1beta converting enzyme (ICE)/Ced-3 family and an upstream regulator of ICE.J. Biol. Chem. 271: 20580-20587.2. Lin, X.Y. et al. (2000) Expression analysis of the human Caspase-1 subfamily reveals specific regulation of the CASP5 gene by Lipopolysaccharide and Interferon-gamma.J. Biol. Chem. 275: 39920-39926.3. Munday, N.A. et al. (1995) Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases.J. Biol. Chem. 270: 15870-15876.4. Cohen, G.M. (1997) Caspases: the executioners of apoptosis.Biochem. J. 326: 1-16.
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