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Anti-UBC13 (C-Terminal) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-UBC13 (C-Terminal) Antibody  ABD11513
    Western blot analysis of human small intestine lysate probed with Rabbit anti Human Ubc13 at 0.5(A), 1(B) and 2(C) ug/ml
  • IHC - Anti-UBC13 (C-Terminal) Antibody  ABD11513
    Immunohistochemical staining of mouse thymus with Rabbit anti Mouse Ubc13
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P61088
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 17138 Da
Additional Information
Other Species Rat,M
Purification Antisera to human UBC13 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by ion exchange chromatography.
Immunogen A 15 amino acid peptide located near the human UBC13 carboxy-terminus.
Shelf Life 18 months from date of despatch.
Gene ID 7334
Other Names Ubiquitin-conjugating enzyme E2 N,, Bendless-like ubiquitin-conjugating enzyme, Ubc13, UbcH13, Ubiquitin carrier protein N, Ubiquitin-protein ligase N, UBE2N, BLU
Target/Specificity Rabbit anti-Human UBC13 antibody recognizes the C-terminal (CT) region of Ubiquitin-conjugating enzyme E2N (UBC13), also known as Bendless-like ubiquitin-conjugating enzyme, Ubiquitin carrier protein N or Ubiquitin-protein ligase N. UBC13 is a ~16kDa enzyme which is an important component of the TLR (Toll-like receptor) and IL-1R signalling pathway. UBC13 is a DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation (Marteinet al.2009).
Preservative & Stabilisers 0.02% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-UBC13 (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Stagg, H.R. et al. (2009) The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.
J Cell Biol. 186: 685-92. 2. Burr, M.L. et al. (2013) MHC class I molecules are preferentially ubiquitinated on endoplasmic reticulum luminal residues during HRD1 ubiquitin E3 ligase-mediated dislocation.
Proc Natl Acad Sci U S A. 110 (35): 14290-5.1. Chen, Z.J. (2005) Ubiquitin signalling in the NF-kappaB pathway.
Nature Cell Biology. 7: 758-766.

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Cat# ABD11513
(40 western blots)
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