|Application ||WB, IHC-P|
|Calculated MW||17138 Da|
|Purification||Antisera to human UBC13 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by ion exchange chromatography.|
|Immunogen||A 15 amino acid peptide located near the human UBC13 carboxy-terminus.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Ubiquitin-conjugating enzyme E2 N, 22.214.171.124, Bendless-like ubiquitin-conjugating enzyme, Ubc13, UbcH13, Ubiquitin carrier protein N, Ubiquitin-protein ligase N, UBE2N, BLU|
|Target/Specificity||Rabbit anti-Human UBC13 antibody recognizes the C-terminal (CT) region of Ubiquitin-conjugating enzyme E2N (UBC13), also known as Bendless-like ubiquitin-conjugating enzyme, Ubiquitin carrier protein N or Ubiquitin-protein ligase N. UBC13 is a ~16kDa enzyme which is an important component of the TLR (Toll-like receptor) and IL-1R signalling pathway. UBC13 is a DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation (Marteinet al.2009).|
|Preservative & Stabilisers||0.02% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-UBC13 (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
1. Stagg, H.R. et al. (2009) The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.J Cell Biol. 186: 685-92. 2. Burr, M.L. et al. (2013) MHC class I molecules are preferentially ubiquitinated on endoplasmic reticulum luminal residues during HRD1 ubiquitin E3 ligase-mediated dislocation.Proc Natl Acad Sci U S A. 110 (35): 14290-5.1. Chen, Z.J. (2005) Ubiquitin signalling in the NF-kappaB pathway.Nature Cell Biology. 7: 758-766.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.
Request Quote Here