|Application ||WB, IHC-P|
|Calculated MW||17138 Da|
|Purification||Antisera to human UBC13 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by ion exchange chromatography.|
|Immunogen||A 15 amino acid peptide located near the human UBC13 carboxy-terminus.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Ubiquitin-conjugating enzyme E2 N, 188.8.131.52, Bendless-like ubiquitin-conjugating enzyme, Ubc13, UbcH13, Ubiquitin carrier protein N, Ubiquitin-protein ligase N, UBE2N, BLU|
|Target/Specificity||Rabbit anti-Human UBC13 antibody recognizes the C-terminal (CT) region of Ubiquitin-conjugating enzyme E2N (UBC13), also known as Bendless-like ubiquitin-conjugating enzyme, Ubiquitin carrier protein N or Ubiquitin-protein ligase N. UBC13 is a ~16kDa enzyme which is an important component of the TLR (Toll-like receptor) and IL-1R signalling pathway. UBC13 is a DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation (Marteinet al.2009).|
|Preservative & Stabilisers||0.02% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-UBC13 (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Stagg, H.R. et al. (2009) The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.J Cell Biol. 186: 685-92. 2. Burr, M.L. et al. (2013) MHC class I molecules are preferentially ubiquitinated on endoplasmic reticulum luminal residues during HRD1 ubiquitin E3 ligase-mediated dislocation.Proc Natl Acad Sci U S A. 110 (35): 14290-5.1. Chen, Z.J. (2005) Ubiquitin signalling in the NF-kappaB pathway.Nature Cell Biology. 7: 758-766.
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