|Application ||WB, IF, E|
|Calculated MW||26886 Da|
|Purification||Antisera to green fluorescent protein (GFP) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Fusion protein corresponding to full length GFP derived from Aequorea victoria|
|Shelf Life||18 months from date of despatch.|
|Other Names||Green fluorescent protein, GFP|
|Target/Specificity||Goat anti-green fluorescent protein antibody recognizes green fluorescent protein (GFP), a 27kD protein which is derived from the jellyfish Aequorea victoria. GFP fluoresces green (509nm) when excited by blue light (395nm) and is commonly used as a marker of gene expression. The antibody recognises wild type GFP, but will also detect recombinant and enhanced forms of GFP.|
|Preservative & Stabilisers||0.01% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Green Fluorescent Protein Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Wen, J, & Brogna, S. (2010) Splicing-dependent NMD does not require the EJC in Schizosaccharomyces pombe.EMBO J. 29: 1537-51. 2. Wuest, T. et al. (2011) The Herpes Simplex Virus-1 Transactivator Infected Cell Protein-4 Drives VEGF-A Dependent Neovascularization.PLoS Pathog. 7: e1002278. 3. Al-Jubran, K. et al. (2013) Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus.RNA. 19: 1669-83.
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