|Calculated MW||43031 Da|
|Purification||Antisera to rat connexin43 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within connexin43 which includes phosphorylated Ser368|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Gap junction alpha-1 protein, Connexin-43, Cx43, Gap junction 43 kDa heart protein, Gja1, Cxn-43|
|Target/Specificity||ABD11515 specifically recognises 43kDa Connexin43, when phosphorylated at Ser368. Connexin43 (CX43) allows for the exchange of solutions between neighbouring cells through the formation of gap-junctional channels and is expressed in the brain, kidneys and myocardium, as well as vascular endothelial cells. Studies suggest that a decrease in the permeability of gap-junctional channels occurs due to a conformational change in the structure of CX43, and that this is mediated by the phosphorylation of CX43 at residue Ser368, by Protein Kinase C (PKC). A possible link between CX43 and epilepsy has been investigated following the detection of high levels of CX43 in both low-grade tumours and peritumoural reactive astrocytes, see Aronica, E. et al. for details.|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Connexin 43 (pSer368) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Bao, X. et al. (2004) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation.Am. J. Physiol. Cell Physiol. 286: C647-C654.2. Bao, X. et al. (2004) Regulation of purified and reconstituted connexin 43 hemichannels by Protein Kinase C-mediated phosphorylation of Serine 368*.J. Biol. Chem. 279: 20058-20066.3. Aronica, E. et al. (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex.Acta. Neuropathol. (Berl). 101: 449-459.
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