|Calculated MW||166071 Da|
|Purification||Antisera to rat NMDA receptor NR2B subunit were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within the NR2B subunit of the NMDA receptor, which includes phosphorylated Tyr1336.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Glutamate receptor ionotropic, NMDA 2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, Grin2b|
|Target/Specificity||ABD11516 specifically recognises the 180kDa NR2B subunit of the NMDA (N-methyl-D-aspartate) receptor when phosphorylated at Tyr1336.Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue Homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+ permeability and the phosphorylation of Tyr1336 is thought to potentiate NMDAR-dependent Ca2+influx.The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer’s disease (Popke, E.J. 2003) and also epilepsy and ischemic neuronal cell death.|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-NMDA Receptor NR2B (pTyr1336) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Takasu, M.A. et al. (2002) Modulation of NMDA receptor-dependent calcium influx and gene expression through EphB receptors.Science. 295: 491-495. 2. Rosenblum, K. et al. (1996) Long-term potentiation increases tyrosine phosphorylation of the N-Methyl-D-Aspartate receptor subunit 2B in rat dentate gyrus in vivo.P.N.A.S USA. 93: 10457-10460.1. Ishii, T. et al. (1993) Molecular characterization of the family of the N-Methyl-D-Aspartate receptor subunits.J. Biol. Chem. 268: 2836-2843.2. Popke, E.J. (2003) From anticholinesterase toxicity to Alzheimer’s disease: Important interactions of cholinergic and NMDA receptor systems.Toxological Sciences 72: 185-187.
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