|Application ||WB, IHC-F|
|Calculated MW||105509 Da|
|Purification||Antisera to rat NMDA receptor, NR1 subunit, splice variant N1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Synthetic peptide from rat NMDA receptor NR1 subunit, containing the N1 splice variant insert.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Glutamate receptor ionotropic, NMDA 1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1, Grin1, Nmdar1|
|Target/Specificity||ABD11521 specifically recognises the 120kDa NR1 subunit of the NMDA (N-methyl-D-aspartate) receptor, containing the N1 splice variant insert. This antibody does not recognize the NR1 subunit, where the N1 splice variant insert is absent. Receptors for NMDA (NMDAR) belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+permeability.The NR1 subunit has eight splice variants, arising from either the deletion or insertion of three exon cassettes within the C-terminal (C1, C2) and N-terminal (N1) NR1 domains. These splice variants differ in both their expression and pharmacological properties.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Prior to reconstitution store at +4℃. Following reconstitution store at -20℃.|
|Precautions||Anti-NMDA Receptor/NR1 Subunit/Splice Variant N1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Ishii, T. et al. (1993) Molecular characterization of the family of the N-Methyl-D-Aspartate receptor subunits.J. Biol. Chem. 268: 2836-2843.2. Ehlers, M.D. et al. (1998) Splice variant-specific interaction of the NMDA receptor subunit NR1 with neuronal intermediate filaments.J. Neurosci. 18: 720-730.
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