|Calculated MW||37337 Da|
|Purification||Antisera to human MCL-1 (IN) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.|
|Immunogen||A 16 amino acid peptide located near the centre of human MCL-1.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl2-L-3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, MCL1, BCL2L3|
|Target/Specificity||ABD11524 recognises an epitope within the central region (IN) of human Myeloid cell leukaemia sequence 1 (MCL-1), an anti-apoptotic member of the Bcl-2 family of proteins, first identified in differentiating ML-1 myeloid cells, and expressed in many different cell lines, tissues and a variety of tumours.The MCL-1 protein, localised to intracellular membranes and particularly mitochondrial membranes, has since been isolated in two distinct isoforms, known as MCL-1 long (MCL-1L) and MCL-1 short (MCL-1S). MCL-1S has been identified as a pro-apoptotic splice variant of MCL-1L, missing the transmembrane and BH1 and BH2 (Bcl-2 homology) domains. Interaction between these two antagonistic variants can act to regulate cell survival.ABD11524 recognises both the MCL-1L (38kDa) and MCL-1S (30kDa) isoforms of MCL-1.|
|Preservative & Stabilisers||0.02% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Mcl-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Kozopas, K.M. et al. (1993) MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2.P.N.A.S USA 90: 3516-3520.2. Bae, J. et al. (2000) MCL-1S, a splicing variant of the antiapoptotic BCL-2 family member MCL-1, encodes a proapoptotic protein possessing only the BH3 domain.J. Biol. Chem. 275: 25255-25261.3. Rinkenberger, J.L. et al. (2000) Mcl-1 deficiency results in peri-implantation embryonic lethality.Genes and Dev. 14: 23-27.
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