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>   home   >   Products   >   Primary Antibodies   >   Anti-Human Dendritic Cells Antibody, clone RFD1    

Anti-Human Dendritic Cells Antibody, clone RFD1

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone Names RFD1
Additional Information
Other Species Macaque
Purification Purified IgM prepared by affinity chromatography
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Human dendritic cells antibody, clone RFD1 recognizes an epitope within the class II MHC complex. In normal tissue mouse anti-human dendritic cells antibody, clone RFD1 identifies dendritic cells and a proportion of B lymphocytes. Within the macrophage/dendritic cell populations the epitope seen by RFD1 is co-expressed with RFD7 by a subset of cells that exhibit suppressive activity on T cell stimulation.Mouse anti-Human dendritic cells antibody, clone RFD1 does not bind to monocytes or cells of the granulocyte lineage.Mouse anti-Human dendritic cells antibody, clone RFD1 has been used for the induction of apoptosis via MHC Class II cross linking of gastric mucosa epithelial cells (Fanet al.1998).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Dendritic Cells Antibody, clone RFD1 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Poulter, L. W. et al. (1986) Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies.
Scand. J. Immunol. 24: 351 - 357. 2. Eckstein, A.K. et al. (2004) Thyroid associated ophthalmopathy: evidence for CD4(+) gammadelta T cells; de novo differentiation of RFD7(+) macrophages, but not of RFD1(+) dendritic cells; and loss of gammadelta and alphabeta T cell receptor expression.
Br J Ophthalmol. 88: 803-8. 3. Hamilton, R.F. Jr. et al. (2004) Alveolar macrophages from systemic sclerosis patients: evidence for IL-4-mediated phenotype changes.
Am J Physiol Lung Cell Mol Physiol. 286: L1202-9. 4. Rugtveit, J. et al. (1997) Differential distribution of B7.1 (CD80) and B7.2 (CD86) costimulatory molecules on mucosal macrophage subsets in human inflammatory bowel disease (IBD)
Clin Exp Immunol. 110: 104–13. 5. Leonard, C.T. et al. (2000) Dendritic cells and macrophages in lung allografts: A role in chronic rejection?
Am J Respir Crit Care Med. 161: 1349-54. 6. Fan, X. et al. (1998) The effect of class II major histocompatibility complex expression on adherence of Helicobacter pylori and induction of apoptosis in gastric epithelial cells: a mechanism for T helper cell type 1-mediated damage.
J Exp Med. 187: 1659-69. 7. Figueroa, D.J. et al. (2000) Expression of the type I diabetes-associated gene LRP5 in macrophages, vitamin A system cells, and the Islets of Langerhans suggests multiple potential roles in diabetes.
J Histochem Cytochem. 48: 1357-68. 8. Kiekens, R.C. et al. (2001) Heterogeneity within tissue-specific macrophage and dendritic cell populations during cutaneous inflammation in atopic dermatitis.
Br J Dermatol. 145: 957-65. 9. Sprinzl, G.M. et al. (1996) Analysis of antigen presenting cells in the human tonsil: A clinical and histopathological study
212, Session G, Podium 10. Pantelidis, P. et al. (2001) Tumour necrosis factor-alpha production in fibrosing alveolitis is macrophage subset specific.
Respir Res. 2: 365-72. 11. Li, T.F. et al. (2002) Dendritic cells in rheumatoid synovial membrane after total removal of the hyaline articular cartilage.
Rheumatology (Oxford). 41: 319-23. 12. Bland, D.A. et al. (2006) H. pylori receptor MHC class II contributes to the dynamic gastric epithelial apoptotic response.
World J Gastroenterol. 12: 5306-10. 13. Fan, X. et al. (2000) Helicobacter pylori urease binds to class II MHC on gastric epithelial cells and induces their apoptosis.
J Immunol. 165: 1918-24.

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Cat# ABD11544
(40 western blots)
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