|Application ||IHC-F, FC, E, IP|
|Calculated MW||57825 Da|
|Purification||Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum|
|Immunogen||Human ICAM-1, domain 1.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, CD54, ICAM1|
|Target/Specificity||Mouse anti-Human CD54 antibody, clone 6.5B5 recognizes human Intercellular adhesion molecule 1, also known as ICAM-1, CD54 or Major group rhinovirus receptor. CD54 is a 532 amino acid, single pass type I transmembrane glycoprotein bearing two Ig-like C2-type domains. CD54 is expressed on activated endothelial cells, epithelial cells, lymphocytes and monocytes.Mouse anti-Human CD54 antibody, clone 6.5B5 blocks LFA-1 mediated adhesion to ICAM-1. Removal of sodium azide is recommended prior to use in functional assays.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD54 Antibody, clone 6.5B5 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Wellicome, S.M. et al. (1990) A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide.J. Immunol. 144: 2558. 2. Thornhill, M.H., Haskard, D.O. (1990) IL-4 regulates endothelial cell activation by IL-1, tumor necrosis factor, or IFN-gamma.J. Immunol. 145: 865-872. 3. Thornhill, M.H. et al. (1990) IL-4 increases human endothelial cell adhesiveness for T cells but not for neutrophils.J. Immunol. 144: 3060-3065. 4. Poston, R.N. et al. (1992) Expression of intercellular adhesion molecule-1 in atherosclerotic plaques.Am. J. Pathol. 140: 665-673. 5. Norris P. et al. (1992) Adhesion molecule expression in polymorphic light eruption.J. Invest. Dermatol. 99: 504. 6. Hamblin, A.S. et al. (1994) Circulating adhesion molecules in sarcoidosis.Clin. Exp. Immunol. 96 335. 7. Brown, D.W. et al. (1999) Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and alphaebeta7-positive lymphocytes to oral and skin keratinocytes.Immunology. 98: 9-15.
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