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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD54 Antibody, clone 6.5B5    

Anti-Human CD54 Antibody, clone 6.5B5

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05362
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 6.5B5
Calculated MW 57825 Da
Additional Information
Purification Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Immunogen Human ICAM-1, domain 1.
Shelf Life 18 months from date of despatch.
Gene ID 3383
Other Names Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, CD54, ICAM1
Target/Specificity Mouse anti-Human CD54 antibody, clone 6.5B5 recognizes human Intercellular adhesion molecule 1, also known as ICAM-1, CD54 or Major group rhinovirus receptor. CD54 is a 532 amino acid, single pass type I transmembrane glycoprotein bearing two Ig-like C2-type domains. CD54 is expressed on activated endothelial cells, epithelial cells, lymphocytes and monocytes.Mouse anti-Human CD54 antibody, clone 6.5B5 blocks LFA-1 mediated adhesion to ICAM-1. Removal of sodium azide is recommended prior to use in functional assays.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD54 Antibody, clone 6.5B5 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Wellicome, S.M. et al. (1990) A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide.
J. Immunol. 144: 2558. 2. Thornhill, M.H., Haskard, D.O. (1990) IL-4 regulates endothelial cell activation by IL-1, tumor necrosis factor, or IFN-gamma.
J. Immunol. 145: 865-872. 3. Thornhill, M.H. et al. (1990) IL-4 increases human endothelial cell adhesiveness for T cells but not for neutrophils.
J. Immunol. 144: 3060-3065. 4. Poston, R.N. et al. (1992) Expression of intercellular adhesion molecule-1 in atherosclerotic plaques.
Am. J. Pathol. 140: 665-673. 5. Norris P. et al. (1992) Adhesion molecule expression in polymorphic light eruption.
J. Invest. Dermatol. 99: 504. 6. Hamblin, A.S. et al. (1994) Circulating adhesion molecules in sarcoidosis.
Clin. Exp. Immunol. 96 335. 7. Brown, D.W. et al. (1999) Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and alphaebeta7-positive lymphocytes to oral and skin keratinocytes.
Immunology. 98: 9-15.

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Cat# ABD11545
(40 western blots)
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