|Application ||FC, E|
|Calculated MW||31438 Da|
|Purification||Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum|
|Immunogen||Spleen and lymph node cells from F344 rats.|
|Shelf Life||18 months from date of despatch.|
|Other Names||T-cell ecto-ADP-ribosyltransferase 2, 22.214.171.124, ADP-ribosyltransferase C2 and C3 toxin-like 2, ARTC2, Alloantigen Rt6.2, Mono(ADP-ribosyl)transferase 2B, T-cell NAD(P)(+)--arginine ADP-ribosyltransferase 2, T-cell mono(ADP-ribosyl)transferase 2, T-cell surface protein Rt6.2, Art2b, Rt6-b|
|Target/Specificity||Rat anti-Rat RT6.2 antibody, clone GY1/12 recognizes rat T-cell ecto-ADP-ribosyltransferase 2, also known as Alloantigen Rt6.2, RT6.2, ADP-ribosyltransferase C2 and C3 toxin-like 2, ARTC2, Mono(ADP-ribosyl) transferase 2B or T-cell surface protein Rt6.2. RT6.2 is a 275 amino acid GPI anchored cell membrane protein belonging to the Arg-specific ADP-ribosyltransferase family. RT6.2 has an N-terminal 20 amino acid signal peptide sequence and 29 amino acid C-terminal propeptide region removed in the mature form.Rt6 is expressed exclusively by post-thymic T cells (Kochet al.1990) with RT6.2 alloantigen being expressed by RT6B rat strains incliuding BH.6B, BS,6B, LEW.6b and F334.6B (Kochet al.1988). Defects in the RT6 gene expression correlate with increased susceptibility for insulin dependent diabetes and other autoimmune diseases (Haaqet al.1996). Rat anti-Rat RT6.2 antibody, clone GY1/12 does not recognize the RT6.1 alloantigen expressed by post-thymic T-cells of RT6A rat strains including BUF, LEW and PVG. The RT6.1 alloantigen is recognized by the Rat anti-Rat RT6.1 antibody, clone P4/16.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat RT6.2 (Peripheral T Cells) Antibody, clone GY1/12 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Butcher, G.W. (1987) A list of monoclonal antibodies specific for alloantigens of the rat.J. Immunogenetics 14: 163-176. 2. Thiele, H.G. Koch, F., Hamann, A. and Arndt, R., (1986) Biochemical characterization of the T-cell alloantigen RT-6.2Immunology 59: 195-201. 3. Koch, F., Haag, F., Kashan, A. and Thiele, H.G., (1990) Primary structure of rat RT6.2, a nonglycosylated phosphatidylinositol-linked surface marker of postthymic T cells.Proc. Natl. Acad. Sci. U.S.A. 87: 964-967. 4. Takada, T., Lida, K. and Moss, J. (1994) Expression of NAD glycohydrolase activity by rat mammary adenocarcinoma cells transformed with rat T cell alloantigen RT6.2.J. Biol. Chem. 269: 9420-9423. 5. O'Sullivan, N.L. et al. (2001) Lymphocyte lineages at mucosal effector sites: rat salivary glands.J Immunol. 166: 5522-9. 6. Bell, E.B. et al. (1989) The origin of T cells in permanently reconstituted old athymic nude rats. Analysis using chromosome or allotype markers.Immunology. 68: 547-56. 7. Orloff, S.L. et al. (2000) Tolerance induced by bone marrow chimerism prevents transplant vascular sclerosis in a rat model of small bowel transplant chronic rejection.Transplantation. 69: 1295-303. 8. Koch, F. et al. (1988) The rat T-cell differentiation marker RT6.1 is more polymorphic than its alloantigenic counterpart RT6.2.Immunology. 65: 259-65. 9. Fangmann, J. et al. (1991) Diabetes-prone BB rats express the RT6 alloantigen on intestinal intraepithelial lymphocytes.Eur J Immunol. 21: 2011-5. 10. Rigby, M.R. et al. (1996) Rat RT6.2 and mouse Rt6 locus 1 are NAD+: arginine ADP ribosyltransferases with auto-ADP ribosylation activity.J Immunol. 156: 4259-65. 11. Fangmann, J. et al. (1991) Unusual phenotype of intestinal intraepithelial lymphocytes in the rat: predominance of T cell receptor alpha/beta+/CD2- cells and high expression of the RT6 alloantigen.Eur J Immunol. 21: 753-60.
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