|Application ||WB, IP|
|Other Accession||Q63787, P23727, P27986|
|Calculated MW||83517 Da|
|Purification||Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum|
|Immunogen||Balculovirus expressed (Recombinant) Bovine p85 alpha subunit of PI3 kinase.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, Pik3r1|
|Target/Specificity||Mouse anti-PI-3 Kinase p85 Subunit alpha antibody, clone U13 recognizes the SH3 domain of the p85 alpha subunit of bovine PI3 kinase, also known as Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha or PtdIns-3-kinase regulatory subunit p85-alpha. p85 α PI3 kinase is a 724 amino acid ~85kDa protein bearing a single Rho-GAP domain, two SH2 domains and a single N-terminal SH3 domain. Multiple isoforms of the protein can be generated by alternative splicing, mouse anti-PI-3 Kinase p85 subunit alpha antibody, clone U13 recognizes only those isoforms containing the SH3 domain (isoforms 1 and 4) but not those lacking the domain (isoforms 2,3 and 5).Deficiencies in the expression of the PIK3R1 gene can lead to immunodeficiency Agammaglobulinemia 7, autosomal recessive, diue to the inhibition of B cell development (Conleyet al.2012) and the develpmental condition SHORT syndrome (Chudasamaet al.2013).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-PI-3 Kinase p85 Subunit alpha Antibody, clone U13 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Woodfield, R. et al. (2001) The p85 subunit of phosphoinositide 3-kinase is associated with beta-catenin in the cadherin-based adhesion complex.Biochem. J. 360: 335 - 344. 2. Reif. K. et al. (1993) Divergent regulation of phosphatidylinositol 3-Kinase P85 alpha and P85 beta isoforms upon T cell activation.J. Biol. Chem. 278:10780-10788. 3. Dawson, C.W. et al. (2003) Epstein-Barr virus latent membrane protein 1 (LMP1) activates the phosphatidylinositol 3-kinase/Akt pathway to promote cell survival and induce actin filament remodeling.J Biol Chem. 278: 3694-704. 4. Cross, M.J. et al. (1995) Wortmannin and its structural analogue demethoxyviridin inhibit stimulated phospholipase A2 activity in Swiss 3T3 cells. Wortmannin is not a specific inhibitor of phosphatidylinositol 3-kinase.J Biol Chem. 270: 25352-5. 5. Gillham, H. et al. (1999) Intracellular movement of green fluorescent protein-tagged phosphatidylinositol 3-kinase in response to growth factor receptor signaling.J Cell Biol. 146: 869-80. 6. Shepherd, P.R. et al. (1997) Differential regulation of phosphoinositide 3-kinase adapter subunit variants by insulin in human skeletal muscle.J Biol Chem. 272: 19000-7.
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