|Application ||WB, IHC-F, FC, E, IP|
|Calculated MW||88415 Da|
|Other Species||Pig,Cynomolgus Pr,Rh|
|Purification||Ig fraction prepared by ammonium sulphate precipitation.|
|Immunogen||Purified Human beta1 integrin|
|Shelf Life||18 months from date of despatch.|
|Other Names||Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29, ITGB1, FNRB, MDF2, MSK12|
|Target/Specificity||Mouse anti-Human CD29 antibody, clone JB1B recognizes human integrin beta1, also known as CD29, Fibronectin receptor subunit beta, Glycoprotein IIa or VLA-4 subunit beta. CD29 is a 798 amino acid ~130 kDa single pass type-1 transmembrane glycoprotein containing a single VWFA domain. Specificity for CD29 has been confirmed with integrin beta1 transfectants and by reciprocal preclearing of beta1 reactivity with anti-beta1 antibody.Mouse anti-Human CD29 antibody, clone JB1B has been successfully employed for the detection of porcine CD29 in formalin fixed, paraffin embedded material (Papageorgiouet al.2014) and also to stimulate the adherence of several cell types (Wilkinset al.1996).The removal of azide is recommended prior to use in functional assays.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD29 Antibody, clone JB1B is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Stupack, D.G. et al. (1994) Control of lymphocyte integrin function: evidence for multiple contributing factors.Cell Immunol. 155 (1): 237-45. 2. Wilkins, J.A. et al. (1996) Control of beta1 integrin function. Localization of stimulatory epitopes.J Biol Chem. 271 (6): 3046-51. 3. Yoshino N et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110. 4. Batista da Silva, A.P. et al. (2004) The major outer sheath protein of Treponema denticola inhibits the binding step of collagen phagocytosis in fibroblasts.Cell Microbiol. 6: 485-98. 5. Levite, M. et al. (2000) Extracellular K(+) and opening of voltage-gated potassium channels activate T cell integrin function: physical and functional association between Kv1.3 channels and beta1 integrins.J Exp Med. 191: 1167-76. 6. Bass, R. et al. (2005) Regulation of urokinase receptor proteolytic function by the tetraspanin CD82.J Biol Chem. 280: 14811-8. 7. Clark, K. et al. (2000) Production of recombinant soluble human integrin alpha4beta1.FEBS Lett. 471: 182-6. 8. Papageorgiou, I. et al. (2014) Interaction of micron and nano-sized particles with cells of the dura mater.J Biomed Mater Res B Appl Biomater. 102 (7): 1496-505. 9. Takaki, T. (2006) Interferon-gamma inhibits collagen phagocytosis in human fibroblasts by inducing subcortical actin assembly and reducing ability of beta1 integrin to bind to collagen.Inflamm Res. 55: 534-42.
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