|Application ||IHC-F, FC|
|Purification||Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum|
|Immunogen||Porcine Lamina Propria Leucocytes|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Pig SWC8, clone MIL3, recognizes the porcine SWC8 cell surface antigen, an antigen that as yet has no identified human homolog. SWC8 is expressed by granulocytes, B cells, a subset of T cells and by some non-haematopoietic cells. Monocytes however do not express SWC8.Clone MIL3 has been used in two colour flow cytometry with clone MIL2 to distinguish between monocytes and granulocytes (Haversonet al.1994).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Pig SWC8 Antibody, clone MIL3 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Haverson K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues.J. Immunol. Methods. 170: 233-245. 2. Saalmuller, A. (1996) Characterization of swine leukocyte differentiation antigens.Immunol. Today 17: 352-354. 3. Summerfield, A. et al. (2001) Induction of apoptosis in bone marrow neutrophil-lineage cells by classical swine fever virus.J. Gen. Virol. 82: 1309-1318. 4. Zelnickova, P. et al. (2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.Vet Immunol Immunopathol. 124: 367-78. 6. Barnard, A.L. et al. (2005) Immune response characteristics following emergency vaccination of pigs against foot-and-mouth disease.Vaccine. 23: 1037-47. 7. Chen, L. et al. (2003) Macrophages and MHC class II positive dendritiform cells in the iris and choroid of the pig.Curr Eye Res. 26: 291-6. 8. Ondrackova, P. et al. (2010) Porcine mononuclear phagocyte subpopulations in the lung, blood and bone marrow: dynamics during inflammation induced by Actinobacillus pleuropneumoniae.Vet Res. 41: 64. 9. Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon-producing cells.Immunology. 110: 440-9.5. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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