|Application ||IHC-F, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Porcine Lamina Propria Leucocytes.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Pig CD11R1, clone MIL4 recognizes the porcine cell surface antigen classified as CD11R1 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haversonet al.2001). Clone MIL4 stains porcine eosinophils, a subset of neutrophils and NK cells, it does not stain monocytes or macrophages (Haversonet al.1994).Mouse anti-Pig CD11R1, clone MIL4 immunoprecipitates a band corresponding to integrin β2 (CD18) of ~95kDa, in common with all other anti-CD11 antibodies tested at the workshop and also a band of ~165 kDa corresponding to CD11R1, in a manner identical to the cross reactive anti-human CD11b clone, TMG6-5 from peripheral blood mononuclear cell lysates suggesting that porcine CD11R1 is analogous to human CD11b (Dominguezet al.2001)Mouse anti-pig CD11R1, clone MIL4 is cross reactive with the guinea pig and is useful for the identification of a population of guinea pig natural killer cells, Kurloff cells (Takizawaet al.2004) (Ereminet al.1980).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Pig CD11R1 Antibody, clone MIL4 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Haverson, K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues.J Immunol Methods. 170 (2): 233-45. 2. Domínguez, J. et al. (2001) Workshop studies on monoclonal antibodies in the myeloid panel with CD11 specificity.Vet Immunol Immunopathol. 80 (1-2): 111-9. 3. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa. J Leukoc Biol. 88: 571-8. 4. Cheng, Q. et al. (2010) Administered CpG oligodeoxynucleotide induces mRNA expression of CXC and CC chemokines at the intestinal mucosa and PBMCs in piglets.Int Immunopharmacol. 10: 611-8. 5. Ordway, D.et al. (2007) The cellular immune response to Mycobacterium tuberculosis infection in the guinea pig.J Immunol. 179: 2532-41. 6. Shang, S. et al. (2010) The activity of TMC 207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.Antimicrob Agents Chemother. 55: 124-31. 8. Rank, R.G. et al. (2012) Effect of Inflammatory Response on In Vivo Competition between Two Chlamydial Variants in the Guinea Pig Model of Inclusion Conjunctivitis.Infect Immun. 80: 612-9. 9. Suda, Y. et al. (2014) Immunobiotic Lactobacillus jensenii as immune-health promoting factor to improve growth performance and productivity in post-weaning pigs.BMC Immunol. 15: 24. 10. Shegarfi, H. et al. (2015) Regulation of CCN1 (Cyr61) in a porcine model of intestinal ischemia/reperfusion.Innate Immun. 21 (5): 453-62.7. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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