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Anti-Horse CD2 Antibody, clone Mac288

Rat Anti-Horse Monoclonal Antibody

  • FC - Anti-Horse CD2 Antibody, clone Mac288  ABD11562
    Staining of equine peripheral blood lymphocytes with Rat anti Equine CD2 , visualised with Rabbit anti Rat IgG:FITC
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P37998
Reactivity Horse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names Mac288
Calculated MW 38864 Da
Additional Information
Immunogen Equine peripheral blood lymphocytes.
Gene ID 100034205
Other Names T-cell surface antigen CD2, CD2, CD2
Target/Specificity Rat anti-Horse CD2 antibody, clone Mac288 recognizes equine CD2, a 347 amino acid, single pass type-1 transmembrane glycoprotein containing single Ig-like C2-typeandIg-like V-typedomains.Equine CD2 is expressed by a large proportion of peripheral blood lymphocytes, most T-cells and few B-cells, it is not present on granulocytes. Immunohistochemical staining shows expression in most T cells of lymph node paracortex and occasional staining of lymphocytes in the follicular areas. In the thymus, rat anti-horse CD2 antibody, clone Mac288 stains the majority of mature thymocytes in the medulla and at least half of the immature thymocytes in the cortex (Kyddet al.1994). Rat anti-Horse CD2 antibody, clone Mac288 recognizes CD2 in Haflinger (Üneret al.2013) and Lusitano horse breeds (Ferreira-Diaset al.2005). Rat anti-Horse CD2 antibody, clone Mac288 is a reliable pan T-cell marker for analysis of cell mediated immunity in horses infected with equine herpes virus by multi-parameter flow cytometry (Plattet al.2010).
PrecautionsAnti-Horse CD2 Antibody, clone Mac288 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Tavernor, A.S. et al. (1994) Expression cloning of an equine T-lymphocyte glycoprotein CD2 cDNA: structure-based analysis of conserved sequence elements.
Eur. J. Biochem. 219: 969-976. 2. Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses.
Can J Vet Res. 71: 213-7. 3. Kydd, J.H. (1989) Maternal immune responses to pregnancy in equids.
PhD Thesis (Cantab). 4. Platt, R. et al. (2010) Cell-mediated immunity evaluation in foals infected with virulent equine herpesvirus-1 by multi-parameter flow cytometry.
Vet Immunol Immunopathol. 135: 275-81. 5. Roberto Da Costa, R.P. et al. (2003) Peripheral blood neutrophil function and lymphocyte subpopulations in cycling mares.
Reprod Domest Anim. 38: 464-9. 6. McCandless, E.E. et al. (2013) Hydrocortisone inhibits IFN-? production in equine, ovine, and bovine PBMCs.
Vet Immunol Immunopathol. 153: 128-33. 7. Üner, A.G. et al. (2013) Blood Levels of Leptin and Lymphocyte Subpopulations in Haflinger Mares on Winter and Summer Solstices.
Animal Health, Prod. and Hyg. 1: 183 - 7

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$ 361.00
Cat# ABD11562
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