|Application ||IHC-F, FC|
|Calculated MW||38864 Da|
|Immunogen||Equine peripheral blood lymphocytes.|
|Shelf Life||18 months from date of despatch.|
|Other Names||T-cell surface antigen CD2, CD2, CD2|
|Target/Specificity||Rat anti-Horse CD2 antibody, clone Mac288 recognizes equine CD2, a 347 amino acid, single pass type-1 transmembrane glycoprotein containing single Ig-like C2-typeandIg-like V-typedomains.Equine CD2 is expressed by a large proportion of peripheral blood lymphocytes, most T-cells and few B-cells, it is not present on granulocytes. Immunohistochemical staining shows expression in most T cells of lymph node paracortex and occasional staining of lymphocytes in the follicular areas. In the thymus, rat anti-horse CD2 antibody, clone Mac288 stains the majority of mature thymocytes in the medulla and at least half of the immature thymocytes in the cortex (Kyddet al.1994). Rat anti-Horse CD2 antibody, clone Mac288 recognizes CD2 in Haflinger (Üneret al.2013) and Lusitano horse breeds (Ferreira-Diaset al.2005). Rat anti-Horse CD2 antibody, clone Mac288 is a reliable pan T-cell marker for analysis of cell mediated immunity in horses infected with equine herpes virus by multi-parameter flow cytometry (Plattet al.2010).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Horse CD2 Antibody, clone Mac288 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Tavernor, A.S. et al. (1994) Expression cloning of an equine T-lymphocyte glycoprotein CD2 cDNA: structure-based analysis of conserved sequence elements.Eur. J. Biochem. 219: 969-976. 2. Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses. Can J Vet Res. 71: 213-7. 3. Kydd, J.H. (1989) Maternal immune responses to pregnancy in equids.PhD Thesis (Cantab). 4. Platt, R. et al. (2010) Cell-mediated immunity evaluation in foals infected with virulent equine herpesvirus-1 by multi-parameter flow cytometry.Vet Immunol Immunopathol. 135: 275-81. 5. Roberto Da Costa, R.P. et al. (2003) Peripheral blood neutrophil function and lymphocyte subpopulations in cycling mares.Reprod Domest Anim. 38: 464-9. 6. McCandless, E.E. et al. (2013) Hydrocortisone inhibits IFN-? production in equine, ovine, and bovine PBMCs.Vet Immunol Immunopathol. 153: 128-33. 7. Üner, A.G. et al. (2013) Blood Levels of Leptin and Lymphocyte Subpopulations in Haflinger Mares on Winter and Summer Solstices.Animal Health, Prod. and Hyg. 1: 183 - 7
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