|Application ||IHC-F, FC|
|Clone Names||HIS24 (6B6)|
|Calculated MW||143269 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Single cell suspension of rat peyers patches.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Receptor-type tyrosine-protein phosphatase C, 18.104.22.168, Leukocyte common antigen, L-CA, T200, CD45, Ptprc|
|Target/Specificity||Mouse anti-Rat CD45R antibody, clone HIS24 recognizes the high molecular weight form of the leucocyte common antigen (LCA) or CD45. CD45R is expressed on mature and precursor B lymphocytes, and to some extent on marginal zone B cells and a small subset of T cells.Mouse anti-Rat CD45R antibody, clone HIS24 is routinely used as a pan-B-cell marker for immunohistochemical studies on brain tissue (Aliet al.2005), pancreas (Homo-Delarcheet al.2006), pulmonary tissue (Foxwellet al.2001) and in flow cytometry on bone marrow cells (Prakapaset al.1993).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat CD45R Antibody, clone HIS24 (6B6) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Kroese, F.G. et al. (1987) B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage-associated antigens.Eur J Immunol. 1987 Jul;17(7):921-8. 2. Ali, S. et al. (2005) Combined immunostimulation and conditional cytotoxic gene therapy provide long-term survival in a large glioma model.Cancer Res. 65: 7194-204. 3. Homo-Delarche, F. et al. (2006) Islet inflammation and fibrosis in a spontaneous model of type 2 diabetes, the GK rat.Diabetes. 2006 Jun;55(6):1625-33. 4. Ma, H. et al. (2007) Features of chronic allograft rejection on rat small intestine transplantation. Pediatr Transplant.Pediatr Transplantation 11:165-72. 5. Prakapas, Z. et al. (1993) Enrichment and characterization of thymus-repopulating cells in stroma-dependent cultures of rat bone marrow.J Cell Sci. 104: 1039-48. 6. Erben, R.G. et al. (1998) Ovariectomy augments B lymphopoiesis and generation of monocyte-macrophage precursors in rat bone marrow.Am J Physiol. 274: E476-83. 7. Horibe, E.K. et al. (2008) Rapamycin-conditioned, alloantigen-pulsed dendritic cells promote indefinite survival of vascularized skin allografts in association with T regulatory cell expansion.Transpl Immunol. 18: 307-18. 8. Ménoret, S. et al. (2013) Generation of Rag1-knockout immunodeficient rats and mice using engineered meganucleases.FASEB J. 27: 703-11. 9. Saiki, T. et al. (2001) In vivo roles of donor and host dendritic cells in allogeneic immune response: cluster formation with host proliferating T cells.J Leukoc Biol. 69: 705-12. 10. Foxwell, A.R. et al. (2001) CD8+ T cells have an essential role in pulmonary clearance of nontypeable Haemophilus influenzae following mucosal immunization.Infect Immun. 69: 2636-42. 11. Shen, J. et al. (1998) Histological characterization and pharmacological control of chronic rejection in xenogeneic and allogeneic heart transplantation.Transplantation. 1998 Sep 27;66(6):692-8.
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