|Application ||IF, FC, Func|
|Calculated MW||81263 Da|
|Purification||Purified IgG prepared by Caprylic Acid Precipitation|
|Immunogen||D10.G4.1 cells (Kayeet al.1984).|
|Shelf Life||18 months from date of despatch.|
|Other Names||Platelet endothelial cell adhesion molecule, PECAM-1, CD31, Pecam1, Pecam, Pecam-1|
|Target/Specificity||Hamster anti-Mouse CD31 monoclonal antibody, clone 2H8 recognizes murine CD31, also known as Platelet endothelial cell adhesion molecule or PECAM-1. CD31 is a ~130kDa single pass type-1 membrane glycoprotein bearing six C2 Ig-like domains, expressed on all continuous endothelium including arteries, veins and non-sinusoidal capillaries. CD31 is also expressed on all haemopoietic lineages with the exception of the erythroid line (Bogenet al.1992) . Hamster anti-mouse CD31, clone 2H8 effectively inhibits transmigration of activated polymorphonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonitis clone 2H8 blocks acute inflammation (Bogenet al.1994). CD31 has also been shown to be critically involved in the sensing of changes in shear stress associated with atherosclerotic lesions and in the associated atherogenesis (Stevenset al.2008).|
|Preservative & Stabilisers||Preservative Free|
|Storage||Store at -20℃ only.|
|Precautions||Anti-Mouse CD31 Antibody, clone 2H8 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Bogen, S.A. et al. (1992) Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.Am J Pathol. 141 (4): 843-54. 2. Xie, Y. & Muller, W.A. (1993) Molecular cloning and adhesive properties of murine platelet/endothelial cell adhesion molecule 1.Proc. Natl. Acad. Sci. 90: 5569-5573. 3. Bogen, S. et al. (1994) Monoclonal antibody to murine PECAM-1 (CD31) blocks acute inflammation in vivo.J. Exp. Med. 179: 1059-1064. 4. Bixel, M.G. et al. (2010) CD99 and CD99L2 act at the same site as, but independently of, PECAM-1 during leukocyte diapedesis.Blood. 116: 1172-84. 5. Ishikawa, J. et al. (2002) Use of anti-platelet-endothelial cell adhesion molecule-1 antibody in the control of disease progression in established collagen-induced arthritis in DBA/1J mice.Jpn J Pharmacol. 88: 332-40. 6. Thurston, G. et al (2005) Angiopoietin 1 causes vessel enlargement, without angiogenic sprouting, during a critical developmental periodDevelopment. 132: 3317-26. 7. Lonsdorf, A.S. et al. (2012) Engagement of aIIbß3 (GPIIb/IIIa) with a?ß3 Integrin Mediates Interaction of Melanoma Cells with Platelets: A CONNECTION TO HEMATOGENOUS METASTASIS.J Biol Chem. 287: 2168-78. 8. Rijcken, E. et al. (2007) PECAM-1 (CD 31) mediates transendothelial leukocyte migration in experimental colitis.Am J Physiol Gastrointest Liver Physiol. 293: G446-52. 9. Vielhauer, V. et al. (2005) Renal cell-expressed TNF receptor 2, not receptor 1, is essential for the development of glomerulonephritis.J Clin Invest. 115: 1199-209. 10. Brackett, C.M. et al. (2013) IL-17 promotes neutrophil entry into tumor-draining lymph nodes following induction of sterile inflammation.J Immunol. 191 (8): 4348-57. 11. Bixel, M.G. et al. (2010) CD99 and CD99L2 act at the same site as, but independently of, PECAM-1 during leukocyte diapedesis.Blood. 116: 1172-84. 12. Wu, Y. et al. (2010) Therapeutic antibody targeting of individual Notch receptors.Nature. 464: 1052-7. 13. Wilhelm, A. et al. (2015) CD248/endosialin critically regulates hepatic stellate cell proliferation during chronic liver injury via a PDGF-regulated mechanism.Gut. pii: gutjnl-2014-308325.
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