|Application ||IHC-P, FC, E, IP|
|Calculated MW||25644 Da|
|Purification||Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum|
|Immunogen||115-130 AA sequence linked to thyroglobulin|
|Shelf Life||18 months from date of despatch.|
|Other Names||Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2|
|Target/Specificity||Mouse anti-Human TNF alpha antibody clone 4C6-H6 recognizes human Tumor necrosis factor, also known as TNF alpha, Cachectin or Tumor necrosis factor ligand superfamily member 2. TNFα is a 233 amino acid ~22kDa type II transmembrane protein with a single potential glycosylation site (Uniprot P01375:Takakura-Yamamotoet al.1996). Mouse anti-Human TNF alpha antibody clone 4C6-H6 has been used successfully for the demonstration of TNFα expression in formalin fixed, paraffin embedded bone marrow biopsies from patients with Myelodysplastic syndrome (Molnáret al.2000) ant has been used for evaluating the increase in TNFα expression seen in peripheral blood mononuclear cells from patients suffering from the autoinnmuse condition, Graves disease (Quadbecket al.2006).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human TNF alpha Antibody, clone 4C6-H6 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Bebôk, Zs., Márkus, B., Németh, P. (1994) Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry.Breast Cancer Res. Treat. 29: 229-235. 2. Molnár, L. et al. (2000) Detection of TNFalpha expression in the bone marRow and determination of TNFalpha production of peripheral blood mononuclear cells in myelodysplastic syndrome. Pathol. Oncol. Res. 6: 18-23. 3. Togo, T. et al. (2001) Glial involvement in the degeneration process of Lewy body-bearing neurons and the degradation process of Lewy bodies in brains of dementia with Lewy bodies.J Neurol Sci. 184: 71-5. 4. Brown, H. et al. (1999) Cytokine expression in the brain in human cerebral malaria.J Infect Dis. 180: 1742-6. 5. Quadbeck, B. et al. (2006) Dysregulation of TNF/TNFR superfamily members: a systemic link between intra- and extrathyroidal manifestations in Graves' disease.Scand J Immunol. 64: 523-30.
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