|Application ||IHC-F, IF, FC, IP|
|Calculated MW||144604 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Purified B cells from mouse lymph nodes.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Receptor-type tyrosine-protein phosphatase C, 18.104.22.168, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5|
|Target/Specificity||Rat anti-Mouse CD45 antibody, clone IBL-3/16 recognizes murine Receptor-type tyrosine-protein phosphatase C, also known as CD45, Leukocyte common antigen, T200 or Lymphocyte antigen 5 (Ly5). CD45 is a 1291 amino acid ~175kDa single pass type I transmembrane glycoprotein belonging to the protein -tyrosing phosphatase family. CD45 has two fibronectin type-III domains and two tyrosine protein phosphatase domains (UniProt:: P06800).Multiple isoforms are generated by alternative splicing with isoforms having differing deletions in the N-terminal region (Saga et al. 1987). Rat anti-Mouse CD45 antibody, clone IBL-3/16 is expected to regognize all isoforms of murine CD45.Rat anti-Mouse CD45 antibody, clone IBL-3/16 has been used sucessfully for the identification of CD45 in murine samples using Immunohistochemical (both cryo and FFPE), immunofluorescence and western blotting techniques (Kondoet al.2011,Cuadroset al.2006).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD45 Antibody, clone IBL-3/16 is for research use only and not for use in diagnostic or therapeutic procedures.|
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1. Clausen, B.H. et al. (2008) Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.J Neuroinflammation. 5: 46. 2. Laporte, V. et al. (2006) CD40 deficiency mitigates Alzheimer's disease pathology in transgenic mouse models.J Neuroinflammation. 3: 3. 3. Paris, D. et al. (2010) Reduction of beta-amyloid pathology by celastrol in a transgenic mouse model of Alzheimer's disease.J Neuroinflammation. 7: 17. 4. Kondo, Y. et al. (2011) Macrophages counteract demyelination in a mouse model of globoid cell leukodystrophy.J Neurosci. 31: 3610-24. 5. Boger, H.A. et al. (2007) Long-term consequences of methamphetamine exposure in young adults are exacerbated in glial cell line-derived neurotrophic factor heterozygous mice.J Neurosci. 27: 8816-25. 6. Boger, H.A. et al. (2009) Minocycline restores striatal tyrosine hydroxylase in GDNF heterozygous mice but not in methamphetamine-treated mice.Neurobiol Dis. 33: 459-66. 7. Cuadros, M.A. et al. (2006) Specific immunolabeling of brain macrophages and microglial cells in the developing and mature chick central nervous system.J Histochem Cytochem. 54: 727-38. 8. Jiang, H.R. et al. (2001) Total dose and frequency of administration critically affect success of nasal mucosal tolerance induction.Br J Ophthalmol. 85: 739-44. 9. Peng, Y. et al. (2010) L-3-n-butylphthalide improves cognitive impairment and reduces amyloid-beta in a transgenic model of Alzheimer's disease.J Neurosci. 30: 8180-9. 10. Richards, J.G. et al. (2003) PS2APP transgenic mice, coexpressing hPS2mut and hAPPswe, show age-related cognitive deficits associated with discrete brain amyloid deposition and inflammation.J Neurosci. 23: 8989-9003. 11. Li, L. et al. (2008) GRK5 deficiency exaggerates inflammatory changes in TgAPPsw mice.J Neuroinflammation. 5: 24. 12. Rahman, A. et al. (2011) Chronic colitis induces expression of ß-defensins in murine intestinal epithelial cells.Clin Exp Immunol. 163: 123-30. 13. Klose, A. et al. (2013) Monocyte/macrophage MMP-14 modulates cell infiltration and T-cell attraction in contact dermatitis but not in murine wound healing.Am J Pathol. 182: 755-64. 14. Ricciardelli, C. et al. (2011) The ADAMTS1 protease gene is required for mammary tumor growth and metastasis.Am J Pathol. 179: 3075-85. 15. Wang, P. et al. (2012) IL-22 signaling contributes to West Nile encephalitis pathogenesis.PLoS One. 7: e44153. 16. Passos, G.F. et al. (2013) The bradykinin B1 receptor regulates Aß deposition and neuroinflammation in Tg-SwDI mice.Am J Pathol. 182: 1740-9. 17. Medeiros, R. et al. (2011) Loss of muscarinic M1 receptor exacerbates Alzheimer's disease-like pathology and cognitive decline.Am J Pathol. 179: 980-91. 18. Ferrer-Martín, R.M. et al. (2015) Microglial Activation Promotes Cell Survival in Organotypic Cultures of Postnatal Mouse Retinal Explants.PLoS One. 10 (8): e0135238.
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