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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Mouse CD45 Antibody, clone IBL-3/16    

Anti-Mouse CD45 Antibody, clone IBL-3/16

Rat Anti-Mouse Monoclonal Antibody

     
  • IF - Anti-Mouse CD45 Antibody, clone IBL-3/16  ABD11579
    Published customer image:Rat anti Mouse CD45 antibody, clone IBL-3/16 used for the detection of microglial cells by immunofluorescence.Image caption:Colocalization of activated microglial cells with Aβ plaques and IL-1β immunoreactivity in the TgAPPsw mice with reduced GRK5. Panels A-C, IF staining of Aβ+ plaques (A, red) and surrounding CD45+ microglial cells (B, green), as well as their merged view (C) in the double mice. Scale bar: 50 μm for panels A-C. Panels D-F, IF staining of Aβ+ plaques (D, red) and surrounding CD11b+ microglial cells (E, green), as well as their merged view (F) in the double mice. Scale bar: 50 μm for panels D-F. Panel G, an example of merged view for CD45 (green) and CD11b/c (clone OX42, red) co-staining of the microglial cells. The image showed that, at least in this particular experimental paradigm, the CD45 antibody stained more specifically for the microglial cell profiles; while the OX42 antibody, in addition to its positive staining of the microglial cells, also non-specifically decorated the plaques. Scale bar: 45 μm. Panel H, an example of merged view for Aβ+ plaques (red) and surrounding IL-1β immunoreactivity (green) in the double mice. Scale bar: 30 μm. Panel I, colocalization of CD45+ microglial cells (green) with IL-1β immunoreactivity (red) in the double mice. Scale bar: 30 μm. Blue indicates reference DAPI staining of nuclei.From:Li et al. Journal of Neuroinflammation 2008 5:24.
  • IF - Anti-Mouse CD45 Antibody, clone IBL-3/16  ABD11579
    Published customer image:Rat anti Mouse CD45 antibody, clone IBL-3/16 used for the detection of microglial cells by immunofluorescence.Image caption:LPS treatment does not modify the distribution of microglial cell and cell viability.A, B: Distribution of microglial cells in control (A, CT) and LPS-treated (B, LPS) retinal explants. Left panels display microglial cells labeled with anti-CD45 antibody, while right panels also show cell nuclei stained with Hoechst. The distribution of microglia in LPS-treated explants is similar to that of untreated explants. Representative images of 3 different explants per condition. Scale bar, 50 μm. C: Bar graph showing that LPS treatment has no significant effect on cell viability in retinal explants. Bars represent mean values ± SEM of 5 explants).From: Ferrer-Martín RM, Martín-Oliva D, Sierra-Martín A, Carrasco M-C, Martín-Estebané M, Calvente R, et al. Microglial Activation Promotes Cell Survival in Organotypic Cultures of Postnatal Mouse Retinal Explants.PLoS ONE 10(8): e0135238.
  • IHC - Anti-Mouse CD45 Antibody, clone IBL-3/16  ABD11579
    Published customer imageRat anti Mouse CD45 antibody, clone IBL-3/16 used for the detection of microglia using immunohistochemistry.Image captionPercentage of microgliosis (mean ± s.e.m) by area in (a) Tg APPsw/CD40 def. mice versus Tg APPsw mice and in (b) Tg PSAPP/CD40 def. mice versus Tg PSAPP mice at 22 to 24 months of age calculated by quantitative image analysis. Post hoc comparison between groups are indicated by the marked bars (* p < 0.05; ** p < 0.01). Representative photographs of brain area in (c) Tg APPsw, (e) Tg APPsw/CD40 def., (d) Tg PSAPP and (f) Tg PSAPP/CD40 def. mice stained with CD45 antibody (each bar represents 0.1 mm).From: Laporte V, Ait-Ghezala G, Volmar CH, Mullan M. CD40 deficiency mitigates Alzheimer&#39;s disease pathology in transgenic mouse models.J Neuroinflammation. 2006 Feb 24;3:3.
  • IF - Anti-Mouse CD45 Antibody, clone IBL-3/16  ABD11579
    Published customer image:Rat anti Mouse CD45 antibody, clone IBL-3/16 used for the detection of microglial cells by immunofluorescence.Image caption:Effects of LPS treatment on retinal explants.A: The release of TNF-&alpha; was significantly increased in explants treated with LPS (LPS, gray bar) with respect to untreated explants (CT, black bar). Bars represent mean values of 12 explants per condition ± SEM. *** indicates significant differences (P
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, IF, FC, IP
Primary Accession P06800
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG1
Clone Names IBL-3/16
Calculated MW 144604 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Purified B cells from mouse lymph nodes.
Shelf Life 18 months from date of despatch.
Other Names Receptor-type tyrosine-protein phosphatase C, 3.1.3.48, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5
Target/Specificity Rat anti-Mouse CD45 antibody, clone IBL-3/16 recognizes murine Receptor-type tyrosine-protein phosphatase C, also known as CD45, Leukocyte common antigen, T200 or Lymphocyte antigen 5 (Ly5). CD45 is a 1291 amino acid ~175kDa single pass type I transmembrane glycoprotein belonging to the protein -tyrosing phosphatase family. CD45 has two fibronectin type-III domains and two tyrosine protein phosphatase domains (UniProt:: P06800).Multiple isoforms are generated by alternative splicing with isoforms having differing deletions in the N-terminal region (Saga et al. 1987). Rat anti-Mouse CD45 antibody, clone IBL-3/16 is expected to regognize all isoforms of murine CD45.Rat anti-Mouse CD45 antibody, clone IBL-3/16 has been used sucessfully for the identification of CD45 in murine samples using Immunohistochemical (both cryo and FFPE), immunofluorescence and western blotting techniques (Kondoet al.2011,Cuadroset al.2006).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse CD45 Antibody, clone IBL-3/16 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Clausen, B.H. et al. (2008) Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.
J Neuroinflammation. 5: 46. 2. Laporte, V. et al. (2006) CD40 deficiency mitigates Alzheimer's disease pathology in transgenic mouse models.
J Neuroinflammation. 3: 3. 3. Paris, D. et al. (2010) Reduction of beta-amyloid pathology by celastrol in a transgenic mouse model of Alzheimer's disease.
J Neuroinflammation. 7: 17. 4. Kondo, Y. et al. (2011) Macrophages counteract demyelination in a mouse model of globoid cell leukodystrophy.
J Neurosci. 31: 3610-24. 5. Boger, H.A. et al. (2007) Long-term consequences of methamphetamine exposure in young adults are exacerbated in glial cell line-derived neurotrophic factor heterozygous mice.
J Neurosci. 27: 8816-25. 6. Boger, H.A. et al. (2009) Minocycline restores striatal tyrosine hydroxylase in GDNF heterozygous mice but not in methamphetamine-treated mice.
Neurobiol Dis. 33: 459-66. 7. Cuadros, M.A. et al. (2006) Specific immunolabeling of brain macrophages and microglial cells in the developing and mature chick central nervous system.
J Histochem Cytochem. 54: 727-38. 8. Jiang, H.R. et al. (2001) Total dose and frequency of administration critically affect success of nasal mucosal tolerance induction.
Br J Ophthalmol. 85: 739-44. 9. Peng, Y. et al. (2010) L-3-n-butylphthalide improves cognitive impairment and reduces amyloid-beta in a transgenic model of Alzheimer's disease.
J Neurosci. 30: 8180-9. 10. Richards, J.G. et al. (2003) PS2APP transgenic mice, coexpressing hPS2mut and hAPPswe, show age-related cognitive deficits associated with discrete brain amyloid deposition and inflammation.
J Neurosci. 23: 8989-9003. 11. Li, L. et al. (2008) GRK5 deficiency exaggerates inflammatory changes in TgAPPsw mice.
J Neuroinflammation. 5: 24. 12. Rahman, A. et al. (2011) Chronic colitis induces expression of ß-defensins in murine intestinal epithelial cells.
Clin Exp Immunol. 163: 123-30. 13. Klose, A. et al. (2013) Monocyte/macrophage MMP-14 modulates cell infiltration and T-cell attraction in contact dermatitis but not in murine wound healing.
Am J Pathol. 182: 755-64. 14. Ricciardelli, C. et al. (2011) The ADAMTS1 protease gene is required for mammary tumor growth and metastasis.
Am J Pathol. 179: 3075-85. 15. Wang, P. et al. (2012) IL-22 signaling contributes to West Nile encephalitis pathogenesis.
PLoS One. 7: e44153. 16. Passos, G.F. et al. (2013) The bradykinin B1 receptor regulates Aß deposition and neuroinflammation in Tg-SwDI mice.
Am J Pathol. 182: 1740-9. 17. Medeiros, R. et al. (2011) Loss of muscarinic M1 receptor exacerbates Alzheimer's disease-like pathology and cognitive decline.
Am J Pathol. 179: 980-91. 18. Ferrer-Martín, R.M. et al. (2015) Microglial Activation Promotes Cell Survival in Organotypic Cultures of Postnatal Mouse Retinal Explants.
PLoS One. 10 (8): e0135238.

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