|Application ||WB, E|
|Calculated MW||85659 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||26 amino acid peptide corresponding to residues 150-176 of human GCR linked to human thyroglobulin.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GRL|
|Target/Specificity||Mouse anti-Human glucocorticoid receptor antibody, clone 8E9 recognizes the human glucocorticoid receptor (GR), also known as Nuclear receptor subfamily 3 group C member 1. Human GR is a 777 amino acid ~97kDa (Moraeset al.2005) member of the NR3 subfamily of nuclear hormone receptors, bearing a single nuclear receptor DNA-binding domain. Multiple isoforms of the human glucocorticoid receptor are generated by either alternative splicing or alternative initiation (UniProt:: P04150).In the absence of bound ligand GRs are located in the cytoplasm and are translocated to the nucleus or mitochondrion following ligation (Rossiniet al.1984). GRs are associated with heat shock proteins in the cytoplasm when ligated to steroid hormone, being disrupted on translocation of the steroid:receptor complex to the nucleus (Tseet al.2011). Mouse anti-Human glucocorticoid receptor antibody, clone 8E9 was raised against a conserved region of the glucocorticoid receptor and recognizes human GR, binding to an epitope between amino acids 167-176 and is therefore expected to bind all described isoforms of the human glucocorticoid receptor.Mouse anti-Human glucocorticoid receptor antibody, clone 8E9 has been used successfully for the identification of human glucocorticoid receptor using flow cytometry (Berkiet al.1998), western blotting (Moraeset al.2005) and immunoprecipitation where it has also been shown to bind to the murine GR (Paul-Clarket al.2003,Bartiset al.2007).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Glucocorticoid Receptor Antibody, clone 8E9 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Berki, T. et al. (1998) Production and flow cytometric application of a monoclonal anti-glucocorticoid receptor antibody. J. Immunol. Methods 214: 19-27. 2. Moraes, L.A. et al. (2005) Ligand-specific glucocorticoid receptor activation in human platelets.Blood.106: 4167-75. 3. Paul-Clark, M.J. et al. (2003) Glucocorticoid receptor nitration leads to enhanced anti-inflammatory effects of novel steroid ligands.J Immunol. 171: 3245-52. 4. Bartis, D. et al. (2007) Intermolecular relations between the glucocorticoid receptor, ZAP-70 kinase, and Hsp-90.Biochem Biophys Res Commun. 354: 253-8. 5. Ouyang, J. et al. (2012) Nuclear HSP90 regulates the glucocorticoid responsiveness of PBMCs in patients with idiopathic nephrotic syndrome.Int Immunopharmacol. 14 (3): 334-40. 6. Bourcier, T. et al.(1999)In vitro effects of dexamethasone on human corneal keratocytes.Invest Ophthalmol Vis Sci. 40 (6): 1061-70. 7. Bourcier T et al. (2000) Regulation of human corneal epithelial cell proliferation and apoptosis by dexamethasone.Invest Ophthalmol Vis Sci. 41 (13): 4133-41. 8. Paul-Clark, M.J. et al. (2003) Glucocorticoid receptor nitration leads to enhanced anti-inflammatory effects of novel steroid ligands.J Immunol. 171 (6): 3245-52.
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