|Application ||WB, IHC-P, IHC-F, E|
|Calculated MW||16618 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G.|
|Immunogen||HB-Xag-Protein A Fusion protein.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Protein X, HBx, Peptide X, pX, X|
|Target/Specificity||Mouse anti-Hepatitis B-X antibody, clone 3F6-G10 recognizes the HB-X antigen of hepatitis virus. The Hepatitis B X antigen is a 154 amino acid ~17 kDa multifunctional protein involved in the development of liver chirrosis and hepatocellular carcinoma (UniProt: P03165).Clone 3F6-G10 was produced by immunization of mice with a"HB-X- Protein A"fusion construct and subsequent screening of hybridoma products against a"HB-X-GST"fusion construct (Marczinovitset al.1997).Mouse anti-Hepatitis B-X antibody, clone 3F6-G10 has been used succesfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins (Pálet al.2001). Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera (Pálet al.2005).Fine epitope mapping by phage library screening indicates that the epitope recognized by Mouse anti-Hepatitis B-X antibody, clone 3F6-G10 lies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA (Pálet al.2003).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Hepatitis B X Antigen Antibody, clone 3F6-G10 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Pál, J. et al. (2001) Immunohistochemical assessment and prognostic value of hepatitis B virus X protein in chronic hepatitis and primary hepatocellular carcinomas using anti-HBxAg monoclonal antibody.Pathol Oncol Res. 7: 178-84. 2. Lei, J.H. et al (2007) Effects of HBV X gene and arsenic trioxide on the expression of p53 in cultured HepG2 cells.Chin Med J 120: 2181-4. 3. Pál, J. et al. (2003) Determination of the fine epitope specificity of an anti-hepatitis B virus X protein monoclonal antibody using microanalytical and molecular biological methods.Mol Immunol. 40: 241-6. 4. Pál, J. et al. (2005) Sandwich type ELISA and a fluorescent cytometric microbead assay for quantitative determination of hepatitis B virus X antigen level in human sera.J Immunol Methods. 306: 183-92. 5. Cheng, P. et al. (2009) Hepatitis B virus X protein (HBx) induces G2/M arrest and apoptosis through sustained activation of cyclin B1-CDK1 kinaseOncol Rep. 22: 1101-7. 6. Chun, E. et al. (2003) Tumor eradication by hepatitis B virus X antigen-specific CD8+ T cells in xenografted nude mice.J Immunol. 170: 1183-90. 7. Pál, J. et al. (2006) Comprehensive regression analysis of hepatitis B virus X antigen level and anti-HBx antibody titer in the sera of patients with HBV infection.Pathol Oncol Res. 12: 34-40.
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