|Application ||WB, FC, E|
|Calculated MW||15410 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Purified natural murine IL-5|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interleukin-5, IL-5, B-cell growth factor II, BCGF-II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, TRF, Il5, Il-5|
|Target/Specificity||Rat anti-mouse IL-5, clone TRFK5recognizesmouse interleukin-5. IL-5 is a regulatory cytokine that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.Rat anti-mouse IL-5, clone TRFK5 may be used to neutralize IL-5 bioactivity in a functional assay (Schumacher.et al.1988) and Abramset al.1992).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse Interleukin-5 Antibody, clone TRFK5 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Schumacher, J.H. et al. (1988) The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbant.J. Immunol. 141:1576-1581. 2. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10and IL-5 in clinical samples.Immunol. Rev. 127: 5-24. 3. Rocken, M.R. et al. (1992) Central role for TCR/CD3 ligation in the differentiation of CD4+ T Cells toward a Th1 or Th2 functional phenotype.J. Immunol. 148:47-54. 4. Betz, M. et al. (1990) Regulation and development of cytochrome c-specific IL-4-producing T cells.J. Immunol. 145:1046-1052. 5. Akdis, C. et al. (1996) Epitope-specific T cell tolerance to phospholipase A2 in bee venom immunotherapy and recovery by IL-2 and IL-15 in vitro.J. Clin. Invest. 98: 1676-1683. 6. Portanova, J. et al. (1995) Rapid and selective induction of blood eosinophilia in guinea pigs by recombinant human interleukin 5.Cytokine. 7: 775 - 783.
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