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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Mouse Kappa Light Chain Antibody, clone OX-20    

Anti-Mouse Kappa Light Chain Antibody, clone OX-20

Rat Anti-Mouse Monoclonal Antibody

     
  • E - Anti-Mouse Kappa Light Chain Antibody, clone OX-20  ABD11589
    Purified Mouse IgG used as coating reagent in a direct ELISA detected with Rat anti Mouse Kappa Light Chain:HRP
  • WB - Anti-Mouse Kappa Light Chain Antibody, clone OX-20  ABD11589
    A Mouse monoclonal antibody of the IgG1 isotype was run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lanes 1 and 5. 1, 0.5 and 0.25 ug of antibody were run in lanes 2, 3 and 4 and 6, 7 and 8 respectively. Protein detection was carried out on lanes 2, 3 and 4 using Goat anti Mouse IgG (H/L):HRP at a dilution of 1/10,000. Detection was carried out on lanes 6, 7 and 8 using Rat anti Mouse Kappa Light Chain:HRP at a dilution of 1/1,000. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure time.
  • WB - Anti-Mouse Kappa Light Chain Antibody, clone OX-20  ABD11589
    HeLa (lanes 2, 5 and 8) and HepG2 (lanes 3, 6 and 9) cell lysates were run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lanes 1, 4 and 7. A Mouse anti Human GAPDH was used as the primary antibody at a dilution of 1/1000. Rat anti Mouse Kappa Light Chain:HRP was used as the secondary antibody at 1/1000 (lanes 2 and 3), 1/5000 (lanes 5 and 6) and 1/10000 (lanes 8 and 9). Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession P01837
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names OX-20
Calculated MW 11778 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Purified mouse IgG
Shelf Life 18 months from date of despatch.
Other Names Ig kappa chain C region, IGKC
Target/Specificity Rat anti-Mouse kappa light chain antibody, clone OX-20 recognizes the kappa light chain of murine immunoglobulins. OX-20 does not recognize mouse lambda light chains or bind to the immunoglobulin heavy chain.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse Kappa Light Chain Antibody, clone OX-20 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Galfre, G. et al. (1979) Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG
Nature 277: 131-133.

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Cat# ABD11589
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