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>   home   >   Products   >   Primary Antibodies   >   Metabolism   >   Anti-Poly(ADP-Ribose) Polymerase-1 Antibody, clone A6.4.12    

Anti-Poly(ADP-Ribose) Polymerase-1 Antibody, clone A6.4.12

Mouse Anti-Human Monoclonal Antibody

     
  • IHC - Anti-Poly(ADP-Ribose) Polymerase-1 Antibody, clone A6.4.12  ABD11591
    Published customer image:Mouse anti poly (ADP-ribose) polymerase 1 antibody, clone A6.4.12 used to immunolocalize PARP-1 expression in normal and Alzheimer's diseased brain by immunohistochemistry on formalin fixed paraffin embedded tissue sections.Image caption:Nucleolar PARP-1 immunoreactivity in AD ranged from absent to dispersed and less intense compared to that of controls. ((a) and (b)) Representative immunostaining with diaminobenzidine (DAB) of human hippocampal pyramidal neurons in CA1 region. (a) Prominent nucleolar staining of PARP-1 (arrows) was seen in most of pyramidal neurons of a control case. (b) The nucleolar staining of PARP-1 ranged from absent (arrowheads) to a more dispersed pattern with less intensity of label (arrows) in pyramidal neurons of an AD case.From: Jianying Zeng, Jenny Libien, Fatima Shaik, Jason Wolk, and A. Iván Hernández,“Nucleolar PARP-1 Expression Is Decreased in Alzheimer's Disease: Consequences for Epigenetic Regulation of rDNA and Cognition,”Neural Plasticity, vol. 2016, Article ID 8987928.
  • IHC-P - Anti-Poly(ADP-Ribose) Polymerase-1 Antibody, clone A6.4.12  ABD11591
    Published customer image:Mouse anti poly polymerase 1 antibody, clone A6.4.12 used to immunolocalize PARP-1 expression in normal and Alzheimer's diseased brain by immunohistochemistry on formalin fixed paraffin embedded tissue sections.Image caption:Nucleolar proteins in hippocampal pyramidal cells are altered in AD. ((a)–(h)) Representative figures show colocalization ((d) and (h), yellow) of fibrillarin ((b) and (f), green) and PARP-1 ((c) and (g), red) in the nucleoli of pyramidal neurons. Control cases exhibit high intensity staining (a–d) compared to AD (e–h) . In AD compared to controls, there is a lower percentage of nucleoli that are both PARP-1(+) and fibrillarin(+) ((f)-(g), arrowhead) in CA1 (see Table 2) and CA4 (see Table 3) pyramidal cells and a higher percentage of nucleoli PARP-1(-)/fibrillarin(+) ((f) and (g), arrow) in CA1 (see Table 2) and CA4 (see Table 3), suggesting that different nucleolar proteins are affected in different ways in AD. Scale bar = 20?μm.From: Jianying Zeng, Jenny Libien, Fatima Shaik, Jason Wolk, and A. Iván Hernández,“Nucleolar PARP-1 Expression Is Decreased in Alzheimer's Disease: Consequences for Epigenetic Regulation of rDNA and Cognition,”Neural Plasticity, vol. 2016, Article ID 8987928.
  • WB - Anti-Poly(ADP-Ribose) Polymerase-1 Antibody, clone A6.4.12  ABD11591
    Western blot analysis of HEK293 whole cell lysate probed with Mouse anti Human poly polymerase-1 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized using chemiluminescence
  • IHC-P - Anti-Poly(ADP-Ribose) Polymerase-1 Antibody, clone A6.4.12  ABD11591
    Published customer image:Mouse anti poly polymerase 1 antibody, clone A6.4.12 used to immunolocalize PARP-1 expression in normal and Alzheimer's diseased brain by immunofluorescence on formalin fixed paraffin embedded tissue sections.Image caption:PARP-1 nucleolar immunoreactivity is altered in hippocampal pyramidal cells in AD brains. Representative confocal microscopy of PARP-1 immunostaining with DAPI nuclear counterstaining of CA4 hippocampal pyramidal neurons. In controls brains (a–c) a high percentage of pyramidal cell nucleoli have intense and well- delineated PARP-1 staining . In contrast, in AD brains (d–f), the percentage of intensely stained and well-delineated nucleoli is less than in the controls and there is a more dispersed pattern with weak label intensity ((d) and (f), arrow). ((g) and (h)) The percentage of CA1 (g) and CA4 (h) hippocampal pyramidal neurons with PARP-1 positive nucleoli staining was less in AD cases compared to controls. (Control, and for CA1 and CA4, resp.; AD, for both CA1 and CA4; .) Scale bar = 25?μM.From: Jianying Zeng, Jenny Libien, Fatima Shaik, Jason Wolk, and A. Iván Hernández,“Nucleolar PARP-1 Expression Is Decreased in Alzheimer's Disease: Consequences for Epigenetic Regulation of rDNA and Cognition,”Neural Plasticity, vol. 2016, Article ID 8987928.
  • WB - Anti-Poly(ADP-Ribose) Polymerase-1 Antibody, clone A6.4.12  ABD11591
    HeLa Nuclear Extract probed with Mouse anti Poly Polymerase
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, E, IP
Primary Accession P09874
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names A6.4.12
Calculated MW 113084 Da
Additional Information
Other Species Ha,M,D,X,Rat
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Immunogen Human PARP-1
Shelf Life 18 months from date of despatch.
Gene ID 142
Other Names Poly [ADP-ribose] polymerase 1, PARP-1, 2.4.2.30, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, PARP1, ADPRT, PPOL
Target/Specificity Mouse anti-poly (ADP-ribose) polymerase 1 antibody, clone A6.4.12 recognizes poly (ADP-ribose) polymerase 1 (PARP-1), a ~116kD nuclear enzyme cleaved during apoptosis (Soldaniet al.2002). PARP-1, a caretaker enzyme, is involved in DNA damage repair (Langelieret al.2013), plays roles in diabetes pathophysiology (Andreoneet al.2012) and tumour proliferation (Rosadoet al2013.).As well as protecting cells from genomic instability, PARP-1 is involved in the development of both inflammatory and immune responses, and cell death by apoptosis and necrosis (Erdélyiet al.2005).Mouse anti-poly(ADP-ribose) polymerase 1 antibody, clone A6.4.12, targets PARP-1, an enzyme which represents a promising target for new developments in therapeutic treatment of immune mediated diseases (Rosadoet al.2013). PARP-1 has considerable potential for delivering selective tumour cell killing while sparing normal cells (Pintonet al.2013).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Poly(ADP-Ribose) Polymerase-1 Antibody, clone A6.4.12 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Harris, J.L. et al. (2009) Aprataxin, poly-ADP ribose polymerase 1 (PARP-1) and apurinic endonuclease 1 (APE1) function together to protect the genome against oxidative damage.
Hum Mol Genet. 18: 4102-17. 2. Freire, R. et al. (2001) Cleavage of the Bloom's syndrome gene product during apoptosis by caspase-3 results in an impaired interaction with topoisomerase IIIalpha.
Nucleic Acids Res. 29 (15): 3172-80. 3. Krohn, A.J. et al. (1998) Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector.
J Neurosci. 18 (20): 8186-97. 4. Staples, C.J. et al. (2010) Cross-talk between the p38alpha and JNK MAPK pathways mediated by MAP kinase phosphatase-1 determines cellular sensitivity to UV radiation.
J Biol Chem. 285 (34): 25928-40. 5. Alexander, B.M. et al. (2010) DNA repair protein biomarkers associated with time to recurrence in triple-negative breast cancer.
Clin Cancer Res. 16: 5796-804. 6. Gueven, N. et al. (2004) Aprataxin, a novel protein that protects against genotoxic stress.
Hum Mol Genet. 1081-93. 7. Gueven, N. et al. (2006) Defective p53 response and apoptosis associated with an ataxia-telangiectasia-like phenotype.
Cancer Res. 66: 2907-12. 8. Kim, J.W. et al. (2000) Inhibition of homodimerization of poly(ADP-ribose) polymerase by its C-terminal cleavage products produced during apoptosis.
J Biol Chem. 275: 8121-5. 9. Staples, C.J. et al. (2010) Cross-talk between the p38alpha and JNK MAPK pathways mediated by MAP kinase phosphatase-1 determines cellular sensitivity to UV radiation.
J Biol Chem. 285: 25928-40. 10. Olaussen, K.A. et al. (2013) PARP1 impact on DNA repair of platinum adducts: Preclinical and clinical read-outs.
Lung Cancer. 80: 216-22. 11. Fabrice, A. et al. (2012) PARP and adjuvant cisplatin-based chemotherapy in non-small-cell lung cancer.
US patent: 20120277110 12. Geistrikh, I. et al. (2011) Ca2+-induced PARP-1 activation and ANF expression are coupled events in cardiomyocytes.
Biochem J. 438: 337-47. 13. Mirzaa, G.M. et al. (2014) Mutations in CENPE define a novel kinetochore-centromeric mechanism for microcephalic primordial dwarfism.
Hum Genet. 133: 1023-39. 14. Milner, R. et al. (2013) Validation of the BRCA1 antibody MS110 and the utility of BRCA1 as a patient selection biomarker in immunohistochemical analysis of breast and ovarian tumours.
Virchows Arch. 462: 269-79. 15. Inbar, D. et al. (2012) Erythropoietin-driven signalling and cell migration mediated by polyADP-ribosylation.
Br J Cancer. 107: 1317-26. 16. Buchsbaum, S. et al. (2012) FAT10 is a proteasomal degradation signal that is itself regulated by ubiquitination.
Mol Biol Cell. 23: 225-32. 17. Mullane, S.A. et al. (2015) Expression Levels of DNA Damage Repair Proteins Are Associated With Overall Survival in Platinum-Treated Advanced Urothelial Carcinoma.
Clin Genitourin Cancer. pii: S1558-7673(15)00359-6. 18. Zeng, J. et al. (2016) Nucleolar PARP-1 Expression Is Decreased in Alzheimer’s Disease: Consequences for Epigenetic Regulation of rDNA and Cognition
Neural Plasticity. 2016: 1-9.1. Pinton, G. et al. (2013) PARP1 inhibition affects pleural mesothelioma cell viability and uncouples AKT/mTOR axis via SIRT1.
J Cell Mol Med. 17: 233-41.2. Rosado, M. et al. (2013) Beyond dna repair,the immunological role of parp-1 and its siblings.
Immunology. 139: 428-37. 3. Andreone, T. et al. (2012) Cytokine-mediatedß-cell damage in PARP-1-deficient islets.
Am J Physiol Endocrinol Metab. 303: E172-9.4. Langelier, M.F. and Pascal, J.M. (2013) PARP-1 mechanism for coupling DNA damage detection to poly(ADP-ribose) synthesis.
Curr Opin Struct Biol. 23: 134-43.

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