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Anti-Human Interleukin-4 Antibody, clone MP4-25D2

Rat Anti-Human Monoclonal Antibody

  • WB - Anti-Human Interleukin-4 Antibody, clone MP4-25D2  ABD11592
    Western blot analysis of human IL-4 recombinant protein probed with Rat anti Human Interleukin-4 followed by F(ab')2 Rabbit anti Rat IgG:HRP
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05112
Reactivity Human
Host Rat
Clonality Monoclonal
Isotype IgG1
Clone Names MP4-25D2
Calculated MW 17492 Da
Additional Information
Other Species Rh
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Immunogen Purified CHO cell expressed recombinant human IL-4.
Shelf Life 18 months from date of despatch.
Gene ID 3565
Other Names Interleukin-4, IL-4, B-cell stimulatory factor 1, BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, IL4
Target/Specificity Rat anti-Human Interleukin-4 antibody, clone MP4-25D2 recognizes human Interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, Binetrakin, Pitrakinra or Lymphocyte stimulatory factor 1. Interleukin-4 is a 153 amino acid ~17kDa cytokine involved in the activation of B cells, macrophages and monocytes (UniProt: P05112).Polymorphisms in the IL-4 gene are asociated with susceptibility to, and outcome of ischemic stroke (Marousiet al.2011).Rat anti-Human Interleukin-4 antibody, clone MP4-25D2 is reported to neutralize IL-4 activity (Abramset al.1992andCarvalhoet al.1994).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Interleukin-4 Antibody, clone MP4-25D2 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Krohn, A. et al. (1998) Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector.
J. Neurosci. 18: 8186-8197. 2. Kubota, M. et al. (1997) Oral immunization with Simian Immunodeficiency Virus p55 gag and cholera toxin elicits both mucosal IgA and systemic IgG immune responses in non human primates.
J. Immunol. 158: 5321-5329. 2. Abrams, J. S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development : Immunoassay of IL-10 and IL-5 in clinical samples.
Immunol. Rev. 127: 5-24. 3. Patterson, B. et al. (2001) Leukemia inhibitory factor inhibits HIV-1 replication and is upregulated in placentae from nontransmitting women.
J. Clin. Invest. 107: 287-294. 5. Carvalho, E. et al. (1994) Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis.
J. Immunol. 152: 5949-5956.

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Cat# ABD11592
(40 western blots)
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