|Application ||WB, IHC-P, IHC-F, E|
|Calculated MW||11737 Da|
|Immunogen||Recombinant human thioredoxin.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Thioredoxin, Trx, ATL-derived factor, ADF, Surface-associated sulphydryl protein, SASP, TXN, TRDX, TRX, TRX1|
|Target/Specificity||Mouse anti-Human thioredoxin antibody, clone 2B1 recognizes human thioredoxin, also known as ATL-derived factor or Surface-associated sulphydryl protein. Thioredoxin is a 105 amino acid ~12kDa dithiol oxidoreductase with powerful protein disulphide oxidoreductase activity containing a single thioredoxin domain (UniProt: P10599). Thioredoxin is involved in a number of cellular processes, including activation of the transcription factor NF-KB (Kelleheret al.2014). Thioredoxin is highly expressed in the glandular cells of the GI tract and uterine tissues, also activated B and T-cells (Rubartelliet al.1992).Mouse anti-Human thioredoxin antibody, clone 2B1 has been used successfully in a biotinylated format as a detection reagent in a thioredoxin sandwich ELISA as part of a study looking at thioredoxin levels in placental trophoblast cells (Di Trapani et al.1998), a study which also used the antibody for immunoprecipitation of thioredoxin as well as detection of thioredoxin by western blotting. Mouse anti-Human thioredoxin antibody, clone 2B1 is also useful for the demonstration of thioredoxin expression on formalin fixed, paraffin embedded material by imunohistochemistry (Lincolnet al.2003). Clone 2B1 has also been used as a capture reagent in a sandwich ELISA for thioredoxin (Perkinget al.1995)|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Thioredoxin Antibody, clone 2B1 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Perkins, A.V. et al. (1995) Immunocytochemical localization of thioredoxin in human trophoblast and decidua.Placenta 16: 635-642. 2. Lincoln, D. et al. (2003) The thioredoxin-thioredoxin reductase system: over-expression in human cancer.Anticancer Res. 23: 2425-2434. 3. Stantchev, T.S. et al. (2012) Cell-type specific requirements for thiol/disulfide exchange during HIV-1 entry and infection.Retrovirology. 9: 97. 4. Ma, X. et al. (2001) Regulation of interferon and retinoic acid-induced cell death activation through thioredoxin reductase.J Biol Chem. 276: 24843-54. 5. Ramanathan, R.K. et al. (2007) A Phase I pharmacokinetic and pharmacodynamic study of PX-12, a novel inhibitor of thioredoxin-1, in patients with advanced solid tumors.Clin Cancer Res. 13: 2109-14. 6. Arnold, N.B. et al. (2004) Thioredoxin is downstream of Smad7 in a pathway that promotes growth and suppresses cisplatin-induced apoptosis in pancreatic cancer.Cancer Res. 64: 3599-606. 7. Kinoshita, T. et al. (2007) Thioredoxin prevents the development and progression of elastase-induced emphysema.Biochem Biophys Res Commun. 354: 712-9. 8. Dorion, S. et al. (2002) Activation of the p38 signaling pathway by heat shock involves the dissociation of glutathione S-transferase Mu from Ask1.J Biol Chem. 277: 30792-7. 9. Sensi, M. et al. (2005) Immunogenicity without immunoselection: a mutant but functional antioxidant enzyme retained in a human metastatic melanoma and targeted by CD8(+) T cells with a memory phenotype.Cancer Res. 65: 632-40. 10. Mamoon, N.M. et al. (2007) Multiple cysteine residues are implicated in Janus kinase 2-mediated catalysis.Biochemistry. 46: 14810-8. 11. Agathanggelou, A. et al. (2003) Identification of Novel Gene Expression Targets for the Ras Association Domain Family 1 (RASSF1A) Tumor Suppressor Gene in Non-Small Cell Lung Cancer and Neuroblastoma.Cancer Res. 63: 5344-51. 12. Di Trapani, G. et al. (1998) Production and secretion of thioredoxin from transformed human trophoblast cells.Mol Hum Reprod. 4: 369-75. 13. Lopata, A. et al. (2001) Expression and localization of thioredoxin during early implantation in the marmoset monkey.Mol Hum Reprod. 7: 1159-65. 14. Stantchev, T.S. et al. (2012) Cell-type specific requirements for thiol/disulfide exchange during HIV-1 entry and infection.Retrovirology. 9: 97.
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