|Application ||WB, IHC-P, IHC-F, FC, IP|
|Calculated MW||26266 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Synthetic peptide, amino acids 41-54 of the Bcl-2 protein|
|Shelf Life||18 months from date of despatch.|
|Other Names||Apoptosis regulator Bcl-2, BCL2|
|Target/Specificity||Mouse anti-Human Bcl-2 antibody, clone 100 recognizes the human Apoptosis regulator Bcl-2 oncoprotein, a 239 amino acid ~25kDa integral single pass membrane protein containing 4 BH motifs which lies within the cell rather than on the cell surface. The protein is localised in the outer mitochondrial membrane and plays a role in the inhibition of apoptosis. Mouse anti-Human Bcl-2, clone 100 reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-haematopoietic tissues few cells are stained (Krajewskiet al.1995). The antibody reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma (Reed 1997).Mouse anti-human Bcl-2, clone 100 has been successfully used to demonstrate Bcl-2 in formalin fixed, paraffin embedded tissues of radioresistant squamous cell carcinoma by immunohistochemistry (Condonet al.2002).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Bcl-2 Antibody, clone 100 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Tsujimoto, Y. & Croce, C.M. (1986) Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma.Proc Natl Acad Sci U S A. 83 (14): 5214-8. 2. Cleary, M.L. et al. (1986) Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation.Cell. 47: 19-28. 3. Pezzella, F. et al. (1990) Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation.Am J Pathol. 137 (2): 225-32. 4. Hockenbery, D. et al. (1990) Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death.Nature. 348 (6299): 334-6. 5. Iannone, F. et al. (2005) Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism.Ann Rheum Dis. 64: 217-21. 6. Iscache, A.L. et al. (2011) Effects of BCL-2 over-expression on B cells in transgenic rats and rat hybridomas.Int Immunol. 23 (10): 625-36. 7. Chylicki, K. et al. (2000) Characterization of the molecular mechanisms for p53-mediated differentiation.Cell Growth Differ. 11: 561-71. 8. Berrieman, H.K. et al. (2005) The expression of Bcl-2 family proteins differs between nonsmall cell lung carcinoma subtypes.Cancer. 103: 1415-9. 9. Valgimigli, M. et al. (2003) Serum from patients with acute coronary syndromes displays a proapoptotic effect on human endothelial cells: a possible link to pan-coronary syndromes.Circulation. 107: 264-70. 10. Shenker, B.J. ,i>et al. (2001) Induction of apoptosis in human T cells by Actinobacillus actinomycetemcomitans cytolethal distending toxin is a consequence of G2 arrest of the cell cycle.J Immunol. 167: 435-41. 11. Fimognari, C. et al. (2002) Growth inhibition, cell-cycle arrest and apoptosis in human T-cell leukemia by the isothiocyanate sulforaphane.Carcinogenesis. 23: 581-6.
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