|Application ||IHC-F, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Bovine WC4 antibody, clone CC55 recognizes the bovine WC4 cell surface antigen, a ~90kDa molecule expressed by a subpopulation of B cells in peripheral blood and lymphoid tissues (Howardet al.1993). It is suggested that WC4 like SWC7 in pigs is the bovine orthologue of human CD19 (Naessens and Howard 1991,Ikebuchiet al.2013).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Bovine WC4 Antibody, clone CC55 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.Vet Immunol Immunopathol. 27 (1-3): 21-7. 2. Denham, S. et al. (1994) Monoclonal antibodies recognising differentiation antigens on porcine B cells.Vet Immunol Immunopathol. 43 (1-3): 259-67. 3. Naessens, J. et al. (1997) Nomenclature and characterization of leukocyte differentiation antigens in ruminants.Immunol Today. 18 (8): 365-8. 4. Boersma, W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers.Vet Immunol Immunopathol. 80 (1-2): 63-78. 5. Andersen, J.K. <et al. (1999) Systematic characterization of porcine ileal Peyer's patch, I. apoptosis-sensitive immature B cells are the predominant cell type.Immunology. 98: 612-21. 7. Szymańska-Czerwińska, M. et al. (2009) Effect of tylosin and prebiotics on the level of cytokines and lymphocyte immunophenotyping parameters in calvesCentral European Journal of Immunology. 34: 1-6. 8. Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro.Vet Res. 44: 59. 9. Ikebuchi, R. et al. (2014) Differences in cellular function and viral protein expression between IgMhigh and IgMlow B-cells in bovine leukemia virus-infected cattle.J Gen Virol. 95: 1832-42.6. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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