|Application ||WB, FC, E, Func|
|Calculated MW||11292 Da|
|Immunogen||Recombinant ovine IL-8.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, CXCL8, IL8|
|Target/Specificity||Mouse anti-Ovine Interleukin-8 antibody, clone 8M6 recognizes ovine interleukin-8 (IL-8), also known as C-X-C motif chemokine 8. IL-8 is a 101amino acid ~16kDa chemoattractant for neutrophils, basophils and T-cells. IL-8 is produced by several cell types including neutrophils, monocytes and macrophages in response to inflammatory stimulation. Mouse anti-ovine Interleukin-8 antibody, clone 8M6 shows no cross-reactivity with ovine IL-1 beta, IL-6, MCP-1 or TNF alpha.Responses to infectious stimuli may vary among ovine species, the response to Mannheimia haemolytica, a causitive agent of pneumonia, peritonitis and gangrenous mastitis in ovids, is exagerrated in Bighorn sheep (Ovis canadensis) compared to domestic sheep (Ovis aries) with significantly elevated IL-8 levels in response to infection (Herndonet al.2010). Mouse anti-ovine interleukin-8 antibody, clone 8M6 has been utilized to identify cells and cell types expressing IL-8 in inflamed porcine tissue (Laursen et al. 2014) showing here also that neutrophils are the predominant cell type expressing IL-8 whilst epithelial and endothelial cells in the vicinity of inflammatory lesions also express the cytokine. Clone 8M6 neutralizes the bioactivity of ovine IL-8. Mouse anti-Ovine Interleukin-8 antibody, clone 8M6 has been used in conjunction with Rabbit anti-Sheep Interleukin-8 antibody (AHP425) for the development of a sensitive ELISA to measuse IL-8 concentrations in feline samples (Croninet al.2014).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Sheep Interleukin-8 Antibody, clone 8M6 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Caswell, J.L. et al. (1998) Expression of the neutrophil chemoattractant interleukin-8 in the lesions of bovine pneumonic pasteurellosis.Vet Pathol. 35: 124-31. 2. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species.Vet Immunol Immunopathol. 88 (3-4): 111-22. 3. Aasted, B. et al. (2002) Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus.Clin Diagn Lab Immunol. 9 (6): 1229-34. 4. Herndon, C.N. et al. (2010) Differential expression of interleukin-8 by polymorphonuclear leukocytes of two closely related species, Ovis canadensisandOvis aries, in response to Mannheimia haemolyticainfection.Infect Immun. 78: 3578-84. 5. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.Vet Immunol Immunopathol. 132:109-15. 6. Zelnickova, P. et al. (2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.Vet Immunol Immunopathol. 124: 367-78. 7. Bonnefont, C.M. et al. (2012) Genetic susceptibility to S. aureus mastitis in sheep: differential expression of mammary epithelial cells in response to live bacteria or supernatant.Physiol Genomics. 44: 403-16. 8. Jensen, P.V. et al. (2003) Cytokine profiles in adult mink infected with Aleutian mink disease parvovirus.J Virol. 77: 7444-51. 9. Singh, B. et al. (2004) Depletion of pulmonary intravascular macrophages inhibits acute lung inflammation.Am J Physiol Lung Cell Mol Physiol. 286: L363-72. 10. Redondo, E. et al. (2013) Induction of Interleukin-8 and Interleukin-12 in Neonatal Ovine Lung Following Experimental Inoculation of Bovine Respiratory Syncytial Virus.J Comp Pathol. 150: 434-48. 11. Laursen, H. et al. (2014) Immunohistochemical detection of interleukin-8 in inflamed porcine tissues.Vet Immunol Immunopathol. 159: 97-102. 12. Boulanger, D. et al. (2003) Increased nuclear factor kappaB activity in milk cells of mastitis-affected cows.J Dairy Sci. 86: 1259-67. 13. Cronin, J.G. et al. (2015) Enzyme linked immunosorbent assay for quantification of bovine interleukin-8 to study infection and immunity in the female genital tract.Am J Reprod Immunol. 73 (4): 372-82.
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