|Application ||WB, IHC-P, IHC-F, IP|
|Calculated MW||43653 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Recombinant human p53.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53|
|Target/Specificity||Mouse anti-Human p53 antibody, clone DO-11 recognizes the human Cellular tumor antigen p53, also known as p53 tumour suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kDa cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells (UniProt: P04637).DO-11 binds to an epitope within the central region of p53 between amino acids 181 - 190 defining a cryptic epitope exposed in unfolded/ denatured p53 (Shimitzuet al.2006). Nine isoforms of human p53 are produced by alternative splicing and promotor usage, the epitope recognized by clone DO-11 is present in all isoforms. Mouse anti-Human p53 antibody, clone DO-11 recognizes mutant forms of p53 (Warnocket al.2011) and has been used successfully for detection of p53 by western blotting (Weiet al.2012).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human p53 (aa181-190) Antibody, clone DO-11 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Vojtěšek, B. et al. (1995) Conformational changes in p53 analysed using new antibodies to the core DNA binding domain of the protein.Oncogene. 10: 389-393. 2. Palecek, E. et al. (2001) Binding of p53 and its core domain to supercoiled DNA.Eur J Biochem. 268: 573-81. 3. Coomber, D.W. and Ward, .R.L. (2001) Isolation of human antibodies against the central DNA binding domain of p53 from an individual with colorectal cancer using antibody phage display.Clin Cancer Res. 7: 2802-8. 4. Wei, J. et al. (2012) Pathogenic bacterium Helicobacter pylori alters the expression profile of p53 protein isoforms and p53 response to cellular stresses.Proc Natl Acad Sci U S A. 109: E2543-50. 5. Warnock, L.J. et al. (2011) Aurora A mediates cross-talk between N- and C-terminal post-translational modifications of p53.Cancer Biol Ther. 12: 1059-68.
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