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Anti-Pig Endothelial Cells Antibody, clone MIL11

Mouse Anti-Pig Monoclonal Antibody

     
  • IF - Anti-Pig Endothelial Cells Antibody, clone MIL11  ABD11627
    Published customer image:Mouse anti Pig Endothelial Cells antibody, clone MIL11 used for the identification of porcine jujenal vasculature by immunofluorescence.Image caption:Colonisation expands IgM and IgA-producing mucosal plasma cells. (A) IgA staining in jejunal mucosa from a GF pig and (B) a colonised pig at 21 days of age. Sections were stained with antibodies to IgA (red) and capillary endothelium (MIL11; in blue). In both (A) and (B), (i) shows the two-colour image; (ii) shows the original greyscale image for the red channel (IgA); (iii) shows the original greyscale image for the blue channel (endothelium). Scale bar represents 10 µm. (C) IgM staining in jejunal mucosa from a GF pig and (D) a colonised pig at 21 days of age. Sections were stained with antibodies to IgM (red). Scale bar represents 10 μm. (E) Area of IgM and (F) IgA staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonised piglets (black squares) at 5 and 21days of age (see Table 1 for numbers of piglets in each experiment). * p
  • IHC - Anti-Pig Endothelial Cells Antibody, clone MIL11  ABD11627
    Published customer image:Mouse anti Pig Endothelial Cells antibody, clone MIL11 used for the identification of endothelium in porcine melanoma by immunofluorescence.Image caption:Expression of collagen IV in swine melanoma. (A) 3-week-old pig. (B) 10-week-old pig. (C) 3-month-old pig. (D) 8-month-old pig. Green: collagen IV; blue: melanin; red: blood vessel walls (A, C) and nuclei (B, D). Bar=20 μm.From: Planska D, Burocziova M, Strnadel J, Horak V. Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.Acta Histochem Cytochem. 2015;48(1):15-26.
  • IF - Anti-Pig Endothelial Cells Antibody, clone MIL11  ABD11627
    Published customer image:Mouse anti Pig Endothelial Cells antibody, clone MIL11 used for the identification of endothelium in porcine melanoma by immunofluorescence.Image caption:Expression of collagen IV in swine melanoma. (A) 3-week-old pig. (B) 10-week-old pig. (C) 3-month-old pig. (D) 8-month-old pig. Green: collagen IV; blue: melanin; red: blood vessel walls (A, C) and nuclei (B, D). Bar=20 μm.From: Planska D, Burocziova M, Strnadel J, Horak V. Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.Acta Histochem Cytochem. 2015;48(1):15-26.
  • IF - Anti-Pig Endothelial Cells Antibody, clone MIL11  ABD11627
    Published customer image:Mouse anti Pig Endothelial Cells antibody, clone MIL11 used for the identification of porcine jujenal vasculature by immunofluorescence.Image caption:Colonisation expands mucosal CD4+ T cells by 21 days of age. (A) CD4 staining in jejunal mucosa from a GF pig and (B) a colonised pig at 21 days of age. Sections were stained with antibodies to CD4 and capillary endothelium (MIL11; in blue). In both (A) and (B), (i) shows the two-colour image; shows the original greyscale image for the blue channel ; shows the original greyscale image for the green channel . Scale bar represents 10 μm. (C) Area of CD4+ T cell staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonised piglets (black squares) at 5 and 21 days of age (see Table 1 for numbers of piglets in each experiment). *p
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F
Reactivity Pig
Host Mouse
Clonality Monoclonal
Isotype IgE
Clone Names MIL11
Additional Information
Immunogen Plastic adherent porcine peripheral blood lymphocytes.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Pig endothelial cells, clone MIL11 raised initially against an immunogen of plastic adherent lymphocytes has been shown to recognize a major subset of porcine endothelial cells. MIL11 was initially identified as staining a characteristic pattern of capillary endothelium in gut forming a network in the lamina propria below the epithelial membrane. Staining was subsequently observed in capillary endothelium in most organs examined, including gut, lung, kidney and skin. Clone MIL11 was observed not to stain endothelium of aorta and other arteries whereas venous endothelium was positive for MIL11 staining. Some population of venous endothelial cells however appear negative for MIL11 staining including those associated with the heart wall, kidney glomeruli and corpus cavernosum (Wilsonet al.1996) .
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Pig Endothelial Cells Antibody, clone MIL11 is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. Wilson, A. D. et al. (1996) Expression of major histocompatibility complex class II antigens, on normal porcine intestinal endothelium.
Immunology 88: 98-103. 2. Harrower, T.P. et al. (2006) Long-term survival and integrationof porcine expanded neural precursor cell grafts in a rat model of Parkinson's disease.
Exp Neurol.197: 56-69. 3. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
J Leukoc Biol. 88 (3): 571-8. 5. Inman, C.F. et al. (2010) Rearing environment affects development of the immune system in neonates.
Clin Exp Immunol. 160: 431-9. 6. Cho, P.S. et al. (2008) Immunogenicity of umbilical cord tissue derived cells.
Blood. 111: 430-8. 7. Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.
PLoS One. 7: e33707. 8. Planska, D. et al. (2015) Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.
Acta Histochem Cytochem. 48 (1): 15-26.4. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.

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Cat# ABD11627
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