|Immunogen||Plastic adherent porcine peripheral blood lymphocytes.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Pig endothelial cells, clone MIL11 raised initially against an immunogen of plastic adherent lymphocytes has been shown to recognize a major subset of porcine endothelial cells. MIL11 was initially identified as staining a characteristic pattern of capillary endothelium in gut forming a network in the lamina propria below the epithelial membrane. Staining was subsequently observed in capillary endothelium in most organs examined, including gut, lung, kidney and skin. Clone MIL11 was observed not to stain endothelium of aorta and other arteries whereas venous endothelium was positive for MIL11 staining. Some population of venous endothelial cells however appear negative for MIL11 staining including those associated with the heart wall, kidney glomeruli and corpus cavernosum (Wilsonet al.1996) .|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Pig Endothelial Cells Antibody, clone MIL11 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Wilson, A. D. et al. (1996) Expression of major histocompatibility complex class II antigens, on normal porcine intestinal endothelium.Immunology 88: 98-103. 2. Harrower, T.P. et al. (2006) Long-term survival and integrationof porcine expanded neural precursor cell grafts in a rat model of Parkinson's disease. Exp Neurol.197: 56-69. 3. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.J Leukoc Biol. 88 (3): 571-8. 5. Inman, C.F. et al. (2010) Rearing environment affects development of the immune system in neonates.Clin Exp Immunol. 160: 431-9. 6. Cho, P.S. et al. (2008) Immunogenicity of umbilical cord tissue derived cells.Blood. 111: 430-8. 7. Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.PLoS One. 7: e33707. 8. Planska, D. et al. (2015) Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.Acta Histochem Cytochem. 48 (1): 15-26.4. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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