|Application ||IHC-F, FC, IP|
|Other Species||Ga,Cat,Mustelid,Pig,B,Mink,Beluga whale|
|Immunogen||Affinity purified beta-2 integrins from splenic lysate|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Dog CD11b antibody, clone CA16.3E10) is a monoclonal antibody recognising the canine CD11b cell surface antigen, a member of the alpha integrin family. CD11b forms one of the possible alpha chains of the canine leukocyte adhesion complexes (LeuCAMs), these contain a common 95 kDa β chain (CD18) non-covalently bound to either a 150 kDa (CD11c), 165 kDa (CD11b) or 180 kDa (CD11a) α chain (Mooreet al.1990. The CD11/CD18 complex is also known as the CR3 receptor.Canine CD11b is expressed by granulocytes, monocytes, NK cells and some macrophages. Mouse anti-Dog CD11b (CA16.3E10) has been used tio evaluate the effect of anaesthetic administration of CD11b expression on canine neutrophils (Maedaet al.2010) demonstrating attenuation of CD11b expression at high concentrations administered lidocaine hydrochloride and reduced adhesion of neutrophils to endothelium.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Dog CD11b Antibody, clone CA16.3E10 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Danilenko, D.M. et al. (1992) Canine leukocyte cell adhesion molecules (LeuCAMs): characterization of the CD11/CD18 family.Tissue Antigens 40: 13-21. 2. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well charaterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.Vet. Immunol. Immunopathol. 64: 1-13. 3. Sampaio, W.M. (2007) In vitro binding and survival assays of Leishmania parasites to peripherical blood monocytes and monocyte-derived macrophages isolated from dogs naturally and experimentally infected with Leishmania chagasi.BMC Vet Res. 3:11. 4. Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.J Neuroinflammation. 9: 226. 5. Yuasa, K. et al. (2007) Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.Gene Ther. 14: 1249-60. 6. Kamstock, D. et al. (2006) Liposome-DNA complexes infused intravenously inhibit tumor angiogenesis and elicit antitumor activity in dogs with soft tissue sarcoma.Cancer Gene Ther. 13: 306-17. 7. Sherger, M. et al. (2012) Identification of myeloid derived suppressor cells in the peripheral blood of tumor bearing dogs.BMC Vet Res. 8: 209. 8. Gregorevic, P. et al. (2009) Evaluation of vascular delivery methodologies to enhance rAAV6-mediated gene transfer to canine striated musculature.Mol Ther. 17: 1427-33. 9. Figueiredo, M.M. et al. (2013) Expression of Toll-like Receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.BMC Immunol. 14: 22. 10. Thompson, L.A. and Romano, T.A. (2015) Beluga (Delphinapterus leucas) granulocytes and monocytes display variable responses to in vitro pressure exposures.Front. Physiol. 6:128.
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