|Application ||IHC-F, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Affinity purified CD11 antigen from canine spleen.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Dog CD11d antibody, clone CA11.8H2 recognizes the canine CD11d cell surface antigen, also known as integrin alpha d subunit.The alpha d integrin (CD11d) subunit is expressed as a heterodimer with beta 2 integrin (CD18). CD11d is expressed by a sub-population of macrophages, some CD8 positive T cells and by gamma/delta T cells in the splenic red pulp.Over expression of CD11d is noted in histiocytic disorders including hemophagocytic sarcoma, described as a proliferative disorder specifically of CD11+ macrophages (Moore.et al.2006) with macrophages demonstrating marked erythrocytic phagocytosis. Previously, canine histiocytic sarcoma were described as originating entirely from interstitial dendritic cells. Cells from animals with canine hepatosplenic T cell lymphoma (HTSL) strongly express CD11d on malignant cells. Dogs normally demonstrate strong positivity for CD11d on large granular lymphocytes, macrophages and T cells in the splenic red pulp. CD11+ γ/δ T cells constitute ~30% red pulp T cells despite their rarity in peripheral blood, suggesting a splenic red pulp origin of HTSL (Fryet al.2003).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Dog CD11d Antibody, clone CA11.8H2 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Danilenko, D.M. et al. (1995) A novel canine leukointegrin, alpha d beta 2, is expressed by specific macrophage subpopulations in tissue and a minor CD8+ lymphocyte subpopulation in peripheral blood.J Immunol. 155:35-44. 2. Moore, P.F. et al. (2006) Canine hemophagocytic histiocytic sarcoma: a proliferative disorder of CD11d+ macrophages.Vet Pathol. 43:632-45. 3. Vernau, W. and Moore, P.F. (1999) An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction.Vet Immunol Immunopathol. 69:145-64. 4. Fry, M.M. et al. (2003) Hepatosplenic lymphoma in a dog.Vet Pathol. 40:556-62. 5. Stokol, T. et al. (2015) Alkaline phosphatase is a useful cytochemical marker for the diagnosis of acute myelomonocytic and monocytic leukemia in the dog.Vet Clin Pathol. 44 (1): 79-93.1. Vernau. W. (2004) Flow Cytometric Assessment of Hematopoietic Neoplasia in the Dog.In:55th Annual Meeting of the American College of Veterinary Pathologists (ACVP) & 39th Annual Meeting of the American Society of Clinical Pathology (ASVCP),
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