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>   home   >   Products   >   Primary Antibodies   >   Anti-Dog CD18 Antibody, clone CA1.4E9    

Anti-Dog CD18 Antibody, clone CA1.4E9

Mouse Anti-Dog Monoclonal Antibody

     
  • IF - Anti-Dog CD18 Antibody, clone CA1.4E9  ABD11636
    Published customer image:Mouse anti Dog CD18 antibody, clone CA1.4E9 used for the evaluation of CD18 expression on alveolar macrophages from hooded seal (Cystophora cristata) using immunofluorescence microscopy.Image caption:Macrophage identification. Adherent BAL cells cultured for 5–7 days were examined for the expression of membrane markers by the use of mAbs. Representative confocal micrographs showing BAL cells immunostained for CD14 (red) and CD18 (green). Nuclei are visualized by DRAQ5 (blue). Pictures are taken of wet samples using a 40X 1.2NA water immersion lens (mAbs; monoclonal antibodies).From: Citation: Larsen AK, Nymo IH, Boysen P, Tryland M, Godfroid J Entry and Elimination of Marine Mammal Brucella spp. by Hooded Seal (Cystophora cristata) Alveolar Macrophages In Vitro.PLoS ONE 8: e70186.
  • FC - Anti-Dog CD18 Antibody, clone CA1.4E9  ABD11636
    Dog peripheral blood monocytes stained with Mouse anti Canine CD18: Alexa Fluor®647
  • FC - Anti-Dog CD18 Antibody, clone CA1.4E9  ABD11636
    Published customer image:Mouse anti Dog CD18 antibody, clone CA1.4E9 used for the evaluation of CD18 expression on alveolar macrophages from hooded seal (Cystophora cristata) using flow cytometry.Image caption:Expression of membrane markers. The expression of CD14 and CD18 on hooded seal PBMC and BAL cells by flow cytometric analysis. PBMCs were divided into monocyte and lymphocyte gates, while BAL cells were broadly gated to encompass all cells of likely macrophage appearance. Only cells classified as viable (using Live/Dead fixable far red stain) were routed to analysis. The respective cell populations were analyzed for CD14 and CD18 (dark grey) or unstained cells (open histograms). For CD18 an indirect staining technique was used, and secondary antibodies alone are depicted in light grey histograms; the resulting dimly positive staining were assumed to be due to unspecific binding, possibly due to Fc receptor cross-reactivity (CD14; cluster of differentiation 14, CD18; cluster of differentiation 18, PBMC; peripheral blood mononuclear cell).From: Citation: Larsen AK, Nymo IH, Boysen P, Tryland M, Godfroid J Entry and Elimination of Marine Mammal Brucella spp. by Hooded Seal (Cystophora cristata) Alveolar Macrophages In Vitro.PLoS ONE 8(7): e70186.
  • FC - Anti-Dog CD18 Antibody, clone CA1.4E9  ABD11636
    Dog peripheral blood monocytes stained with Mouse anti Dog CD18 followed by FITC conjugated Rabbit F(ab')2 anti Mouse IgG
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, IF, FC, IP
Reactivity Dog
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names CA1.4E9
Additional Information
Other Species Hs,Pig,H,B,Cat,Mustelid,Mink,Hooded Seal
Purification Purified IgG prepared by affinity chromatography on Protein G
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Dog CD18 antibody, clone CA1.4E9 recognises the canine CD18 cell surface antigen, also known as the b2 integrin.The tissue and cellular distribution of CD18 in canine tissue closely follows that observed for humans (Mooreet al.1990) CD18 is expressed by virtually all leucocytes, but more strongly upon monocytes and granulocytes than on lymphocytes. The cross reactivity patterns of Mouse anti-Canine CD18, clone CA1.4E9 further indicate that the antibody clone recognizes an epitope common to a number of mammalian species.Immunoprecipitation experiments using detergent lysatesof iodinated peripheral blood leukocytes indicate that clone Ca1.4E9 immunoprecipitates the common 95 kDa beta 2 integrin chain (CD18) along with the non-covalently associated alpha chains at 180 kDa (CD11a ), 165 kDa (CD11b) and 150 kDa (CD11c) (Mooreet al.1990). Of note is the lack of precipitation of the integrin alpha D chain (CD11d), this is likely due to the almost complete absence of CD11d expression on peripheral blood lymphocytes, in contrast to expression in the splenic red pulp (Fryet al.2003). High CD18 expression is a common feature of lymphoma and hyperplasia in dogs (Caniattiet al.1996)
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Dog CD18 Antibody, clone CA1.4E9 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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References

1. Moore, P.F. et al. (1990) Canine leukocyte integrins: characterization of a CD18 homologue.
Tissue Antigens. 36 (5): 211-20. 2. Danilenko, D.M. et al. (1992) Canine leukocyte cell adhesion molecules (leuCAMS): characterization of the CD11/CD18 family.
Tissue Antigens 40: 13-21. 3. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterised monoclonal antibodies with specificities against various leucocyte surface antigens of humans and 11 animal species.
Vet. Immunol. Immunopathol. 64: 1-13. 4. Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression.
Vet Res. 39: 50. 5. Bauer, T.R. Jr. et al. (2006) Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy.
Blood. 108: 3313-20. 6. Leite, F. et al. (2000) Recombinant bovine interleukin-1beta amplifies the effects of partially purified Pasteurella haemolytica leukotoxin on bovine neutrophils in a beta(2)-integrin-dependent manner.
Infect Immun. 68: 5581-6. 7. McDonough, S.P. and Moore, P.F. (2000) Clinical, hematologic, and immunophenotypic characterization of canine large granular lymphocytosis
Vet Pathol. 37: 637-46. 8. Mortarino, M. et al. (2010) Identification of suitable endogenous controls and differentially expressed microRNAs in canine fresh-frozen and FFPE lymphoma samples.
Leuk Res. 34: 1070-7. 9. Donahue, R.E. et al. (2011) Leukocyte integrin activation mediates transient neutropenia after G-CSF administration.
Blood. 118: 4209-14. 10. Larsen, A.K. et al. (2013) Entry and Elimination of Marine Mammal Brucella spp. by Hooded Seal (Cystophora cristata) Alveolar Macrophages In Vitro.
PLoS One. 8: e70186. 11. Comazzi, S. et al. (2006) Flow cytometric expression of common antigens CD18/CD45 in blood from dogs with lymphoid malignancies: a semi-quantitative study.
Vet Immunol Immunopathol. 112 (3-4): 243-52. 12. Yeh, C.L. et al. (2006) Dietary arginine enhances adhesion molecule and T helper 2 cytokine expression in mice with gut-derived sepsis.
Shock. 25 (2): 155-60. 13. Comazzi, S. et al. (2006) Flow cytometric patterns in blood from dogs with non-neoplastic and neoplastic hematologic diseases using double labeling for CD18 and CD45.
Vet Clin Pathol. 35 (1): 47-54. 14. Moreira, M. L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes
Veterinary Parasitology. 15 Feb [Epub ahead of print]1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.

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