|Application ||WB, FC, E, ELISPOT, Func|
|Calculated MW||37050 Da|
|Other Species||H,African Buffalo|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Recombinant bovine IL-12.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40, IL-12 subunit p40, IL12B|
|Target/Specificity||Mouse anti-Bovine Interleukin-12/23 antibody, clone CC301 recognizes the p40 subunit of bovine interleukin-12, and binds to both the free subunit and the intact heterodimer. The p40 subunit is also known as IL-12B and can form a heterodimer with either IL-12A or IL-23A.This antibody may be used as a capture antibody in an ELISA in combination with biotinylated clone CC326 as a detection reagent.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Store at -20℃ only.|
|Precautions||Anti-Bovine Interleukin-12/23 Antibody, clone CC301 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Hope, J. C. et al. (2002) Development of detection methods for ruminant interleukin (IL) - 12. J. Immunol. Methods. 266: 117 - 126. 2. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.J Dairy Sci. 93: 2458-70. 3. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. colimastitis.Funct Integr Genomics. 10: 21-38. 4. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.Vet Res. 42:19. 5. Contreras, V. et al. (2010) Existence of CD8a-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species. J Immunol. 185: 3313-25. 6. Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.Clin Diagn Lab Immunol. 11: 463-72. 7. Davis, T.L. and Pate, J.L. (2007) Bovine luteal cells stimulate proliferation of major histocompatibility nonrestricted gamma delta T cells.Biol Reprod. 77: 914-22. 8. Ferret-Bernard, S. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.PLoS One. 5: e13705. 9. Shoda, L.K. et al. (2001) DNA from protozoan parasites Babesia bovis, Trypanosoma cruzi, and T. brucei is mitogenic for B lymphocytes and stimulates macrophage expression of interleukin-12, tumor necrosis factor alpha, and nitric oxide.Infect Immun. 69: 2162-71. 10. Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus.J Virol. 82: 1777-86. 11. Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.BMC Vet Res. 10: 293. 12. Beechler BR et al. (2015) Enemies and turncoats: bovine tuberculosis exposes pathogenic potential of Rift Valley fever virus in a common host, African buffalo (Syncerus caffer).Proc Biol Sci. 282 (1805): . 13. Pomeroy B et al. (2015) Monocyte-derived dendritic cells from late gestation cows have an impaired ability to mature in response to E. coli stimulation in a receptor and cytokine-mediated fashion.Vet Immunol Immunopathol. 167 (1-2): 22-9.
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