|Application ||WB, IHC-F, E|
|Calculated MW||18323 Da|
|Immunogen||Bovine Myelin Basic protein (MBP) (45-91) / Ovalbumin Conjugate|
|Shelf Life||18 months from date of despatch.|
|Other Names||Myelin basic protein, MBP, 20 kDa microtubule-stabilizing protein, Myelin A1 protein, MBP|
|Target/Specificity||Mouse anti-Human MBP antibody, clone 10 recognizes the synthetic peptide 82-91 and human and bovine 45-91 but not with whole native MBP molecule (Martensen numbering). This epitope is located in the C terminal region of 82-91 and the corresponding epitope is not present (or exposed) in whole MBP.The clone was selected using synthetic human MBP peptide 82-91 for screening (Groome, N.P. et al1985). Since this work was done a sequence error has been described in the human MBP sequence in this region (Gibson,et al.1984).Although the clone was selected to have a high affinity for 82-91 it appears that this antibody reacts more strongly with the unnatural sequence, rather than the correct human 82-91. In consequence the antibody is not a suitable reagent for sensitive immunoassays of correct human 82-91 as was thought at the time the original paper on this antibody was published.The 91-92 phe-phe bond is believed to be cleaved by demyelinating lesions by Cathepsin D to generate peptides ending in the phe 91 which should react with this monoclonal.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-MBP (aa82-91) Antibody, clone 10 (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Groome, N.P., Harland, J. and Dawkes, A. (1985) Preparation and characterisation of monoclonal antibodies to myelin basic protein and its peptides.Neurochemistry International. 7: 309-317. 2. Martensen, R.W. (1984) in Experimental Allergic Encephalomyelitis.A useful model for multiple sclerosis (Alvord E.C., Kies, M.W. and Suckling, A.J. eds). pp 511-521 Alan Liss N.Y. 3. Gibson, B.W., Gilliom, R.D., Whitaker, J.N. and Bieman, K. (1984) Amino acid sequence of human myelin basic protein peptide 45-89 as determined by mass spectrometry.J. Biol. Chem. 259: 5028-5031. 4. Sakuma, H. et al. (2006) Quantitation of myelin oligodendrocyte glycoprotein and myelin basic protein in the thymus and central nervous system and its relationship to the clinicopathologic features of autoimmune encephalomyelitis.J Neurosci Res. 84: 606-13.
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