|Application ||IHC-F, FC|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Pig CD45RC, clone MIL5 recognizes a restricted CD45 epitope encoded by the CD45 C exon and will bind and immunoprecipitate both the 210kDa RC and 226kDa RAC isoforms.The antibody has been reported to be suitable for detection of CD45RC in Western blots. MIL5, binds to an epitope alternative to that bound by clone MIL15, also against CD45RC differing in their ability to stain B cells in tissue sections (Zuckerman, F. A. et al. 2001).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Pig CD45RC Antibody, clone MIL5 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Zuckermann, F. A. et al. (1998) Definition of the specificity of monoclonal antibodies against porcine CD45 and CD45R: report from the CD45/CD45R and CD44 subgroup of the Second International Swine CD workshop.Vet. Immunol. Immunopathol. 60: 367-387. 2. Schnitzlein, W.M. and Zuckermann, F.A. et al. (1998) Determination of the specificity of CD45 and CD45R monoclonal antibodies through the use of transfected hamster cells producing individual porcine CD45 isoforms.Vet. Immunol. Immunopathol. 60: 389-401. 3. Haverson, K. et al. (1999) T-cell populations in the pig intestinal lamina propria: memory cells with unusual phenotypic characteristics.Immunology 96: 66-73. 4. Zuckerman, F. A. et al. (2001) Characterisation of monoclonal antibodies assigned to the CD45 subgroup of the third international swine CD workshop. Vet. Immunol. Immunopathol. 80 (1-2): 165 - 74. 5. Terzic, S. et al. (2002) Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs.Vet Res Commun. 26: 273-83. 6. Bozic, F. et al. (2002) Recruitment of intestinal CD45RA+ and CD45RC+ cells induced by a candidate oral vaccine against porcine post-weaning colibacillosis.Vet Immunol Immunopathol. 86: 137-46.7. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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