|Application ||WB, IHC-P, IHC-F|
|Calculated MW||101896 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Bacterially expressed human Mcm2.|
|Shelf Life||18 months from date of despatch.|
|Other Names||DNA replication licensing factor MCM2, 188.8.131.52, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, MCM2, BM28, CCNL1, CDCL1, KIAA0030|
|Target/Specificity||Mouse anti-Human MCM2 antibody, clone CRCT2.1 recognizes human DNA replication licensing factor MCM2, also known as Mcm2, Nuclear protein BM28 or mini chromosome maintenance protein-2. Mcm-2 is a 904 amino acid ~125kDa nuclear protein involved in the control of DNA replication.Mouse anti-Human MCM2 antibody, clone CRCT2.1 has been used successfully for the detection of human MCM2 in ovarian adenocarcinoma by immunohistochemistry on formalin fixed, paraffin embedded tissues (Gakiopoulouet al.2007).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human MCM2 Antibody, clone CRCT2.1 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Gakiopoulou, H. et al. (2007) Minichromosome maintenance proteins 2 and 5 in non-benign epithelial ovarian tumours: relationship with cell cycle regulators and prognostic implications. Br J Cancer. 97:1124-34. 2. Korkolopoulou, P. et al. (2005) Minichromosome maintenance proteins 2 and 5 expression in muscle-invasive urothelial cancer: a multivariate survival study including proliferation markers and cell cycle regulators.Hum Pathol. 36: 899-907. 3. Giaginis, C. et al. (2009) Clinical significance of MCM-2 and MCM-5 expression in colon cancer: association with clinicopathological parameters and tumor proliferative capacity.Dig Dis Sci. 54: 282-91. 4. Polo, S.E. et al. (2010) Clinical significance and prognostic value of chromatin assembly factor-1 overexpression in human solid tumours.Histopathology. 57: 716-24. 5. FitzGerald, J. et al. (2014) A high through-put screen for small molecules modulating MCM2 phosphorylation identifies Ryuvidine as an inducer of the DNA damage response.PLoS One. 9: e98891. 6. Almouzni, G. et al. (2011) Proliferation markers in clinical practice and their use for breast cancer prognosis or diagnosis.U.S. Patent Application: US 20110003706 A1
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