|Application ||WB, E|
|Calculated MW||16318 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||CHO expressed recombinant ovine GM-CSF.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF, CSF2|
|Target/Specificity||Mouse anti-Sheep GM-CSF, clone 3C2 specifically recognises both recombinant and native ovine Granulocyte-macrophage colony-stimulating factor, also known GM-CSF or CSF, a 16.3kDa cytokine responsible for the stimulation and growth of cells from granulocyte, macrophage, eosinophil and erythrocyte hematopoietic precursor cell linages.Mouse anti-Sheep GM-CSF, clone 3C2 has is of particular interest as it has been shown to reconise GM-CSF in cattle while other clones such as 8D8 do not crosss react with cattle..Mouse anti-Sheep GM-CSF, clone 3C2 has been reported to neutralise the activity of both recombinant ovine and native GM-CSF. Removal of sodium azide is recommended prior to use in functional assays. AbD Serotec recommend the use of EQU003 for this purpose.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Sheep GM-CSF Antibody, clone 3C2 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Entrican, G. et al. (1996) Development of a sandwich ELISA for ovine granulocyte/macrophage colony-stimulating factor.Vet. Immunol. Immunopathol. 50: 105-115. 2. Deane, D. et al. (2000) Orf virus encodes a novel secreted protein inhibitor of granulocyte-macrophage colony-stimulating factor and interleukin-2.J Virol. 74: 1313-20. 3. Foulon, E. and Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.J Immunol Methods. 339: 1-10. 4. Haig, D.M. et al. (1995) Haemopoietic cell responses in the blood and bone marrow of sheep infected with the abomasal nematode Telodorsagia circumcincta.J Comp Pathol. 112: 151-64. 5. Scott, J.L. (2009) Spermatozoa and seminal plasma induce a greater inflammatory response in the ovine uterus at oestrus than dioestrus.Reprod Fertil Dev. 21: 817-26. 6. John, H.A. et al. (1994) Generation of an ovine bone marrow-derived myelomonocyte-like cell line by retroviral-mediated transformation. Immunological characterization and the effect of cytokines and lipopolysaccharides.J Leukoc Biol. 55: 785-92. 7. Kennedy, H.E. et al. (2002) Modulation of immune responses to Mycobacterium bovis in cattle depleted of WC1(+) gamma delta T cells.Infect Immun. 70: 1488-500. 8. McInnes, C.J. et al. (2005) Glycosylation, disulfide bond formation, and the presence of a WSXWS-like motif in the orf virus GIF protein are critical for maintaining the integrity of Binding to ovine granulocyte-macrophage colony-stimulating factor and interleukin-2.J Virol. 79: 11205-13.
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