|Application ||WB, E|
|Calculated MW||16318 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||CHO expressed recombinant ovine GM-CSF.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF, CSF2|
|Target/Specificity||Mouse anti-Sheep GM-CSF, clone 3C2 specifically recognises both recombinant and native ovine Granulocyte-macrophage colony-stimulating factor, also known GM-CSF or CSF, a 16.3kDa cytokine responsible for the stimulation and growth of cells from granulocyte, macrophage, eosinophil and erythrocyte hematopoietic precursor cell linages.Mouse anti-Sheep GM-CSF, clone 3C2 has is of particular interest as it has been shown to reconise GM-CSF in cattle while other clones such as 8D8 do not crosss react with cattle..Mouse anti-Sheep GM-CSF, clone 3C2 has been reported to neutralise the activity of both recombinant ovine and native GM-CSF. Removal of sodium azide is recommended prior to use in functional assays. AbD Serotec recommend the use of EQU003 for this purpose.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Sheep GM-CSF Antibody, clone 3C2 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
1. Entrican, G. et al. (1996) Development of a sandwich ELISA for ovine granulocyte/macrophage colony-stimulating factor.Vet. Immunol. Immunopathol. 50: 105-115. 2. Deane, D. et al. (2000) Orf virus encodes a novel secreted protein inhibitor of granulocyte-macrophage colony-stimulating factor and interleukin-2.J Virol. 74: 1313-20. 3. Foulon, E. and Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.J Immunol Methods. 339: 1-10. 4. Haig, D.M. et al. (1995) Haemopoietic cell responses in the blood and bone marrow of sheep infected with the abomasal nematode Telodorsagia circumcincta.J Comp Pathol. 112: 151-64. 5. Scott, J.L. (2009) Spermatozoa and seminal plasma induce a greater inflammatory response in the ovine uterus at oestrus than dioestrus.Reprod Fertil Dev. 21: 817-26. 6. John, H.A. et al. (1994) Generation of an ovine bone marrow-derived myelomonocyte-like cell line by retroviral-mediated transformation. Immunological characterization and the effect of cytokines and lipopolysaccharides.J Leukoc Biol. 55: 785-92. 7. Kennedy, H.E. et al. (2002) Modulation of immune responses to Mycobacterium bovis in cattle depleted of WC1(+) gamma delta T cells.Infect Immun. 70: 1488-500. 8. McInnes, C.J. et al. (2005) Glycosylation, disulfide bond formation, and the presence of a WSXWS-like motif in the orf virus GIF protein are critical for maintaining the integrity of Binding to ovine granulocyte-macrophage colony-stimulating factor and interleukin-2.J Virol. 79: 11205-13.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.