|Shelf Life||18 months from the date of despatch.|
|Target/Specificity||Mouse anti-Pig CD203a, clone PM18-7 recognizes porcine CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop (Dominguezet al.1998) and later identified as the porcine homologue of human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 or NPP1(Petersenet al.2007).Mouse anti-Pig CD203a was originally reported to immunoprecipitate two bands, one of >205 kDa and one of ~130 kDa (Dominguezet al.1998) under both reducing and non-reducing conditions. Subsequent studies suggest that CD203a migrates as a homodimer of ~260 kDa under non-reducing conditions and a 130 kDa monomer under reducing conditions (Petersenet al.2007) from preparations of porcine alveolar macrophages.CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages (Petersenet al.2007), it is not expressed on monocyte populations (McCulloughet al.1997).SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation (Sanchezet al.1999).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||CD203a Antibody, clone PM18-7 (Monoclonal Antibody) (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. McCullough, K. C. et al (1997) Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages.Vet Immunol Immunopathol 58: 265 - 75. 2. McCullough, K. C. et al (1999) Intermediate stages in monocyte-macrophage differentiation modulate phenotype and susceptibility to virus infection. Immunology 98: 203 - 12. 3. Boersma, W. et al. (2001) Summary of workshop findings for porcine B cell markers. Vet Immunol Immunopathol. 80: 63 - 78. 4. Dominguez, J. et al. (1998) Porcine myelomonocytic markers: summary of the second international swine CD workshop. Vet Immunol Immunopathol. 60: 329 - 341. 5. Dominguez, J. et al. (1998) Workshop studies with monoclonal antibodies identifying a novel porcine differentation antigen SWC9. Vet Immunol Immunopathol. 60: 343 - 349. 6. Petersen, C.B. et al. (2007) Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a):cloning, transcription, expression, mapping, and identification of anNPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies.Dev Comp Immunol. 31: 618 - 631. 7. Basta, S. et al. (1999) Modulation of monocytic cell activity and virus susceptibility during differentiation into macrophages.J Immunol. 162: 3961 - 9. 8. Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.Vet Res. 42: 9. 9. Sanchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.J Immunol. 162: 5230 - 7. 10. Cantu, E. et al. (2006) Depletion of pulmonary intravascular macrophages prevents hyperacute pulmonary xenograft dysfunction.Transplantation 81: 1157 - 64. 11. Basta, S. et al. (2001) Lipopolysaccharide and phorbol 12-myristate 13-acetate both impair monocyte differentiation, relating cellular function to virus susceptibility.Immunology 103: 488 - 97. 13. Lithgow, P. et al. (2014) Correlation of cell surface marker expression with African swine fever virus infection.Vet Microbiol. 168: 413 - 9. 14. Ondrackova, P. et al. (2013) Phenotypic characterisation of the monocyte subpopulations in healthy adult pigs and< i>Salmonella-infected piglets by seven-colour flow cytometry.Res Vet Sci. 94: 240 - 5. 12. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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