|Application ||WB, IHC-F, IP|
|Calculated MW||36938 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A/G|
|Immunogen||Recombinant cdk6 protein|
|Shelf Life||18 months from date of despatch.|
|Other Names||Cyclin-dependent kinase 6, 220.127.116.11, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE, CDK6, CDKN6|
|Target/Specificity||Mouse anti-Human Cdk6 antibody, clone DCS-83 recognizes the human cyclin dependent kinase 6, also known as Cdk6 or Serine/threonine-protein kinase PLSTIRE. Cdk6 is a 326 amino acid member of the CDC2/CDKX subfamily containing a single protein kinase domain. Pck6 migrates at ~37kDa in Western blotting under reducing conditions. Cdk6 pays an important role in cell cycle progression from the G1 to S phase (Sunet al.2008)Mouse anti-Human Cdk6, clone DCS83 additionally recognizes both the rat and mouse homologs of Human Cdk6. and has been successfully used for immunoprecipitation and immunofluorescesnce on acetone/methanol fixed cell preparations (Lukaset al.1999).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Cdk6 Antibody, clone DCS-83.1 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Lukas, C. et al. (1999) Immunohistochemical analysis of the D-type cyclin-dependent kinases Cdk4 and Cdk6, using a series of monoclonal antibodies.Hybridoma. 18: 225-34. 2. Tsubari, M. et al. (1999) Hepatocyte growth factor releases mink epithelial cells from transforming growth factor beta1-induced growth arrest by restoring Cdk6 expression and cyclin E-associated Cdk2 activity.Mol Cell Biol. 19: 3654-63.1. Elledge, S.J. (1996) Cell Cycle Checkpoints: Preventing an identity crisis.Science 274: 1664-1672.
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