|Application ||WB, IHC-P, E|
|Calculated MW||149715 Da|
|Immunogen||Denatured human kidney CD143 (Angiotensin converting enzyme).|
|Shelf Life||18 months from date of despatch.|
|Other Names||Angiotensin-converting enzyme, ACE, 3.2.1.-, 22.214.171.124, Dipeptidyl carboxypeptidase I, Kininase II, CD143, Angiotensin-converting enzyme, soluble form, ACE, DCP, DCP1|
|Target/Specificity||Mouse anti-Human CD143 antibody, clone 3C5 recognizes human CD143, also known as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a ~170 kDa somatic form and a ~90 kDa germinal form. The somatic form is expressed by endothelial cells (especially those of arterioles and lung capillaries), epithelial cells (especially in proximal renal tubules and in the small intestine), by some neuronal cells and variably on some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.Study of CD143 may be relevant to fertility (especially sperm - egg interactions), atherosclerosis and in drug targeting to the lung.Mouse anti-Human CD143 antibody, clone 3C5 has been reported to recognise ACE in western Blotting (Balyasnikovaet al.2003). The antibody recognises denatured ACE, binding to an epitope in the C domain. Mouse anti-Human CD143 antibody, clone 3C5 was developed in the same fusion as clone 2E2 (ABD11686) and binds to a different epitope within domain C. Clone 2E2 is useful for the recognition of denatured CD143 in rodent species.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD143 Antibody, clone 3C5 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Metzger, R. et al. (1999) Angiotensin-converting enzyme in non-neoplastic kidney diseases.Kidney Int. 56 (4): 1442-54. 2. Balyasnikova, I. V. et al. (2003) Monoclonal antibodies to denatured ACE (CD143) broad species specificity, reactivity on paraffin sections and detection of subtle conformational changes in the C-terminal domain of ACE. Tissue Antigens. 61 (1): 49- 62.
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