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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Anti-Human Cytochrome P450 Aromatase Antibody, clone H4    

Anti-Human Cytochrome P450 Aromatase Antibody, clone H4

Mouse Anti-Human Monoclonal Antibody

     
  • FC - Anti-Human Cytochrome P450 Aromatase Antibody, clone H4  ABD11692
    Published customer image:Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in human synovial tissue by immunohistochemistry on cryosectionsImage caption:Aromatase expression in synovial tissue and endogenous steroid hormone release from superfused synovium. (a) Immunohistochemistry of aromatase in one OA and one RA patient. Using the respective control antibody revealed no staining of positive cells (not shown). Magnification: 400×. (b) Density of aromatase-positive cells in OA (open bars, n = 20) and RA patients (hatched bars, n = 16). (c) Spontaneously released E2, E3, and free testosterone from standardized superfused pieces of synovial tissue of OA (open bars, n = 20) and RA patients (hatched bars, n = 18). (b,c) Values are given as box blots with the 5th, 25th, 50th (median), 75th, and 95th percentile when applicable. OA, osteoarthritis; RA, rheumatoid arthritis. Other abbreviations are as given in the legend to Fig. 1.From: Schmidt M, Weidler C, Naumann H, Anders S, Schölmerich J, Straub RH. Androgen conversion in osteoarthritis and rheumatoid arthritis synoviocytes--androstenedione and testosterone inhibit estrogen formation and favor production of more potent 5alpha-reduced androgens. Arthritis Res Ther. 2005;7:R938-48.
  • IHC-P - Anti-Human Cytochrome P450 Aromatase Antibody, clone H4  ABD11692
    Published customer image:Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in sheep tissues by immunohistochemistry on paraffin sectionsImage caption:a and b) Low- (a) and higher- (b) power sections of the fetal end of a placentome in G1 stained with the prolactin antiserum and showing the intensely stained binucleate cells scattered along the trophoblast, but with a tendency to be more densely accumulated towards the fetal end (scale bars a=200? µm; b=150? µm). (c) High-power section of the trophoblast–endometrium interface in a placentome in G2 stained with the prolactin antiserum. Note the patchy staining of the thin, elongated endometrial epithelial cells in close contact with the intensely stained binucleate trophoblast cells (scale bar=40? µm). (d) Low-power section of the intercotyledonary allantochorion stained with the prolactin antiserum and showing the relatively dense accumulation of binucleate trophoblast cells which remain unstained in this region of the placenta (scale bar=100? µm). (e) High-power section at the edge of a placentome of G1 showing positive staining of the uninucleate trophoblast by the 3ß-HSD antiserum. The binucleate cells remain unstained (scale bar=40? µm). (f) Section of the distended endometrial glands at the lateral border of a placentome in G2 stained with 3ß-HSD. The basal portions of the endometrial cells lining the glands stain strongly as does the secretory material adhered to the luminal surface of the cells and the accumulated coagulum, possibly as a result of ‘stickiness’ of the material (scale bar=150? µm). (g) High-power section of the intercotyledonary region of the allantochorion in G1 showing positive staining of the cytoplasm of the uninucleate, but not binucleate trophoblast by the 17,20 lyase antiserum (scale bar=40? µm). (h) Section at the fetal end of a placentome from G2 remaining unstained by the aromatase antiserum (scale bar=150? µm). (i) Section of near-term sheep placenta showing strong, positive staining of the uninucleate, but not binucleate, trophoblast by the aromatase antiserum (scale bar=150? µm). (j) High-power section at the fetal end of a placentome in G1 showing strong positive staining of the uninucleate trophoblast by the PR antiserum. The binucleate cells remain unstained (scale bar=40? µm).From: Wilsher S, Stansfield F, Greenwood RE, Trethowan PD, Anderson RA, Wooding FB, Allen WR. Ovarian and placental morphology and endocrine functions in the pregnant giraffe (Giraffa camelopardalis).Reproduction. 2013 May 21;145(6):541-54.
  • WB - Anti-Human Cytochrome P450 Aromatase Antibody, clone H4  ABD11692
    Published customer image:Mouse anti Human cytochrome p450 aromatase used for the detection of rat aromatase protein by western blotting.Image caption:1,25D3 attenuated testosterone-induced aromatase expression but improved 17ß-estradiol secretions. Ovarian granulosa cells from rats were pretreated with 1,25D3 (0.1 µM) for 15 min and subsequently treated with 0.1 µg/mL or 1 µg/mL testosterone (denoted by T) or vehicle (denoted by -) for 24 h. (A) Cells were lysed for protein extraction, and cell lysates were subjected to western blot analysis for aromatase protein detection. The level of aromatase (denoted by Arom) expression was estimated by densitometric analyses after normalization with the ß-actin signal (n?=?5–6 in each group). (B and C) 17ß-Estradiol secretions from the granulosa cells were collected from the medium and assessed over the course of a 24-h period of drug treatments. 17ß-Estradiol release data were expressed as the fold-change relative to the vehicle-treated control (n?=?4–5 in each group). The values represent the means?±?SEM. **, p?
  • IF - Anti-Human Cytochrome P450 Aromatase Antibody, clone H4  ABD11692
    Published customer image:Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in porcine spermatozoa by immunofluorescenceImage caption:Immunofluorescence labelling of androgen and estrogen receptors in pig spermatozoa: A) AR red fluorescence in sperm proximal mid-piece. B) P450arom red brilliant light in the proximal tail of sperm with a diffuse labelling in the distal tail. C) ERa red fluorescence in the sperm mid-piece, together with a faint labelling in the tail. D) ERß green intense light in the sperm acrosomal region. Inserts: immunonegative absorption controls. Scale bars: 5 µm.From: Rago V, Aquila S, Panza R, Carpino A. Cytochrome P450arom, androgen and estrogen receptors in pig sperm. Reprod Biol Endocrinol. 2007 Jun 6;5:23.
  • IHC-P - Anti-Human Cytochrome P450 Aromatase Antibody, clone H4  ABD11692
    Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in human lung tumors by immunohistochemistry on formalin fixed paraffin embedded tissue.Image caption:Representative immunohistochemistry of lung tumors for ERa, ERß, aromatase, EGFR and PR.From: Stabile LP, Dacic S, Land SR, Lenzner DE, Dhir R, Acquafondata M, Landreneau RJ, Grandis JR, Siegfried JM. Combined analysis of estrogen receptor beta-1 and progesterone receptor expression identifies lung cancer patients with poor outcome. Clin Cancer Res. 2011 Jan 1;17(1):154-64.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession P11511
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names H4
Calculated MW 57883 Da
Additional Information
Other Species Rat,Minke whale,Bryde's whale,Sei whale,B,Hs,Great fruit eating bat,Rb,Sh,Collared peccary
Immunogen Synthetic peptide corresponding to amino acids 376 - 390 of human aromatase.
Shelf Life 18 months from date of despatch.
Gene ID 1588
Other Names Aromatase, 1.14.14.14, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase, CYP19A1, ARO1, CYAR, CYP19
Target/Specificity Mouse anti-Human Cytochrome P450 Aromatase antibody, clone H4 recognizes a conserved epitope within cytochrome P450 aromatase (P450 arom). P450 arom plays an important role in estrogen biosynthesis and is highly conserved amongst mammals.P450 arom is highly expressed in placental tissue. For tissues where there may be low expression of P450 arom, the use of microsomal extracts may improve the staining for Western blots using Mouse anti-Human Cytochrome P450 Aromatase antibody, clone H4 (Turneret al.2002).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Cytochrome P450 Aromatase Antibody, clone H4 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Turner, K. J. et al. (2002) Development and validation of a new monoclonal antibody to mammalian aromatases.
J. Endocrinol. 172: 21-30. 2. Lanzino, M. et al. (2013) DAX-1, as an androgen-target gene, inhibits aromatase expression: a novel mechanism blocking estrogen-dependent breast cancer cell proliferation.
Cell Death Dis. 4: e724. 3. Zhao, D. et al. (2010) Somatic sex identity is cell autonomous in the chicken.
Nature. 464: 237-42. 4. Sirianni, R. et al. (2009) Inhibition of cyclooxygenase-2 down-regulates aromatase activity and decreases proliferation of Leydig tumor cells.
J Biol Chem. 284: 28905-16. 5. Carpino, A. et al. (2007) Detection of aromatase and estrogen receptors (ERalpha, ERbeta1, ERbeta2) in human Leydig cell tumor.
Eur. J. Endocrinol. 157: 239-244. 6. Catalano, S. et al. (2010) Farnesoid X receptor, through the binding with steroidogenic factor 1-responsive element, inhibits aromatase expression in tumor Leydig cells.
J Biol Chem. 285: 5581-93. 7. Wu, Y.G. et al. (2011) Testosterone, not 5{alpha}-Dihydrotestosterone, Stimulates LRH-1 Leading to FSH-Independent Expression of Cyp19 and P450scc in Granulosa Cells.
Mol Endocrinol. 25: 656-68. 8. Lu, Y. et al. (2011) BRCA1/BARD1 complex interacts with steroidogenic factor 1--A potential mechanism for regulation of aromatase expression by BRCA1.
J Steroid Biochem Mol Biol. 123: 71-8. 9. Barone, I. et al. (2009) Expression of the K303R estrogen receptor-alpha breast cancer mutation induces resistance to an aromatase inhibitor via addiction to the PI3K/Akt kinase pathway.
Cancer Res. 69: 4724-32. 10. Ghosh, S. et al. (2008) A Role of CREB in BRCA1 Constitutive Promoter Activity and Aromatase Basal Expression.
Int J Biomed Sci. 4: 260-265. 11. Jeong, J.H. et al. (2010) The gene for aromatase, a rate-limiting enzyme for local estrogen biosynthesis, is a downstream target gene of Runx2 in skeletal tissues.
Mol Cell Biol. 30: 2365-75. 12. Rago, V. et al. (2005) Cytochrome P450 aromatase expression in human seminoma.
Reprod Biol Endocrinol. 3: 72. 13. Schmidt, M. et al. (2005) Androgen conversion in osteoarthritis and rheumatoid arthritis synoviocytes--androstenedione and testosterone inhibit estrogen formation and favor production of more potent 5alpha-reduced androgens.
Arthritis Res Ther. 7: R938-48. 14. Stabile, L.P. et al. (2012) Prevention of Tobacco Carcinogen-Induced Lung Cancer in Female Mice Using Anti-Estrogens.
Carcinogenesis. 33: 2181-9. 15. Oki, Y. et al. (2012) Dedifferentiated follicular granulosa cells derived from pig ovary can transdifferentiate into osteoblasts.
Biochem J. 447: 239-48. 16. Campbell, B.K. et al. (2012) The role of anti-Müllerian hormone (AMH) during follicle development in a monovulatory species (sheep).
Endocrinology. 153: 4533-43. 17. Wilsher, S. et al. (2013) Ovarian and placental morphology and endocrine functions in the pregnant giraffe (Giraffa camelopardalis).
Reproduction. 145: 541-54. 18. Beyer, C. et al. (1994) Androgens influence sexual differentiation of embryonic mouse hypothalamic aromatase neurons in vitro.
Endocrinology. 135: 1220-6. 19. Grzesiak, M. et al. (2012) Elevated level of 17ß-estradiol is associated with overexpression of FSHR, CYP19A1, and CTNNB1 genes in porcine ovarian follicles after prenatal and neonatal flutamide exposure.
Theriogenology. 78: 2050-60. 20. Pakarainen, T. et al. (2005) Knockout of luteinizing hormone receptor abolishes the effects of follicle-stimulating hormone on preovulatory maturation and ovulation of mouse graafian follicles.
Mol Endocrinol. 19: 2591-602. 21. Rago, V. et al. (2007) Cytochrome P450arom, androgen and estrogen receptors in pig sperm
Reprod Biol Endocrinol. 5: 23. 22. Pannetier, M. et al. (2006) FOXL2 activates P450 aromatase gene transcription: towards a better characterization of the early steps of mammalian ovarian development.
J Mol Endocrinol. 36: 399-413. 23. Fazleabas, A.T. et al. (2003) Steroid receptor and aromatase expression in baboon endometriotic lesions.
Fertil Steril. 80: 820-7. 24. Mlodawska, W. and Slomczynska, M. (2010) Immunohistochemical localization of aromatase during the development and atresia of ovarian follicles in prepubertal horses.
Theriogenology. 74: 1707-12. 25. Castro, B. et al. (2013) Bisphenol A exposure during adulthood alters expression of aromatase and 5a-reductase isozymes in rat prostate.
PLoS One. 8: e55905. 26. Oliveira, R.L. et al. (2012) Seasonal variation in estrogen receptor ERa, but not ERß, androgen receptor and aromatase, in the efferent ductules and epididymis of the big fruit-eating bat Artibeus lituratus.
Gen Comp Endocrinol. 179: 1-13. 27. Hanoux, V. et al. (2003) Differential regulation of two 3' end variants of P450 aromatase transcripts and of a new truncated aromatase protein in rabbit preovulatory granulosa cells.
Endocrinology. 144: 4790-8. 28. Mayor, P. et al. (2006) Ovarian features of the wild collared peccary (Tayassu tajacu) from the northeastern Peruvian Amazon.
Gen Comp Endocrinol. 147: 268-75. 29. Gallet, C. et al. (2011) The infusion of glucose in ewes during the luteal phase increases the number of follicles but reduces oestradiol production and some correlates of metabolic function in the large follicles.
Anim Reprod Sci.127: 154-63. 30. Pannetier, M. et al. (2006) FOXL2 activates P450 aromatase gene transcription: towards a better characterization of the early steps of mammalian ovarian development.
J Mol Endocrinol. 36: 399-413. 31. Mann, G.E. et al. (2007) Identification of elevated concentrations of estradiol in bovine uterine endometrium.
Domest Anim Endocrinol. 33: 437-41. 32. Garcia-Morales, C. et al. (2015) Cell-Autonomous Sex Differences in Gene Expression in Chicken Bone Marrow-Derived Macrophages.
J Immunol. pii: 1401982. 33. Lee, C.T. et al. (2014) 1,25-dihydroxyvitamin D3 increases testosterone-induced 17beta-estradiol secretion and reverses testosterone-reduced connexin 43 in rat granulosa cells.
Reprod Biol Endocrinol. 12: 90. 34. Scaramuzzi, R.J. et al. (2015) The effects of intravenous, glucose versus saline on ovarian follicles and their levels of some mediators of insulin signalling.
Reprod Biol Endocrinol. 13 (1): 6. 35. Kim, S.O. et al. (2014) Prostaglandin E2 (EP) receptors mediate PGE2-specific events in ovulation and luteinization within primate ovarian follicles.
Endocrinology. 155 (4): 1466-75. 36. Kato N et al. (2015) Expression of P450 Aromatase in Granulosa Cell Tumors and Sertoli-Stromal Cell Tumors of the Ovary: Which Cells Are Responsible for Estrogenesis?
Int J Gynecol Pathol. Jul 9. [Epub ahead of print] 37. Kitayama C et al. (2015) Structure and functions of the placenta in common minke (Balaenoptera acutorostrata), Bryde's (B. brydei) and sei (B. borealis) whales.
J Reprod Dev. 61 (5): 415-21.

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