Anti-Human Placental Alkaline Phosphatase Antibody, clone H17E2
Mouse Anti-Human Monoclonal Antibody
|Application ||IHC-F, FC, E|
|Calculated MW||57954 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Alkaline phosphatase, placental type, 22.214.171.124, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALPP, PLAP|
|Target/Specificity||Mouse anti-Human placental alkaline phosphatase antibody, clone H17E2 recognizes human placental alkaline phosphatase, also known as Alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type or Placental alkaline phosphatase 1. Placental alkaline phosphatase is a 535 amino acid ~67 kDa GPI-anchored transmembrane glycoprotein bearing a 22 amino acid signal peptide and a 29 amino acid pro-peptide region, cleaved to produce the mature form. Placental alkaline phosphatase is expressed in trophoblast cells of normal human placenta (Traverset al.1984), in seminomas (Langeet al.1982)and in ovarian carcinoma (Tholanderet al.1990).Mouse anti-Human placental alkaline phosphatase antibody, clone H17E2 has been widely used in immunolocalization studies of germ cell tumours and may also be used for the measurement of soluble placental alkaline phosphatase by ELISA.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Placental Alkaline Phosphatase Antibody, clone H17E2 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Travers, P. & Bodmer, W. (1984) Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast-associated determinants.Int J Cancer. 33 (5): 633-41. 2. Tucker, D.F. et al. (1985) Serum marker potential of placental alkaline phosphatase-like activity in testicular germ cell tumours evaluated by H17E2 monoclonal antibody assay.Br J Cancer. 51 (5): 631-9. 3. Epenetos, A.A. et al. (1984) An immunohistological study of testicular germ cell tumours using two different monoclonal antibodies against placental alkaline phosphatase.Br J Cancer. 49 (1): 11-5. 4. Kalofonos, H.P. et al. (1990) Immunolocalisation of testicular tumours using radiolabelled monoclonal antibody to placental alkaline phosphatase.J Nucl Med Allied Sci. 34 (4): 294-8. 5. Long, M.A. & Rossi, F.M. (2009) Silencing inhibits Cre-mediated recombination of the Z/AP and Z/EG reporters in adult cells. PLoS One. 4:e5435. 6. Carter, C.C. et al. (2010) HIV-1 infects multipotent progenitor cells causing cell death and establishing latent cellular reservoirs.Nat Med. 16: 446-51. 7. Wonderlich, E.R. et al. (2011) ADP Ribosylation Factor 1 Activity Is Required To Recruit AP-1 to the Major Histocompatibility Complex Class I (MHC-I) Cytoplasmic Tail and Disrupt MHC-I Trafficking in HIV-1-Infected Primary T Cells.J Virol. 85: 12216-26. 8. Semenov, O.V. et al. (2010) Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.Am J Obstet Gynecol. 202:193.e1-193.e13. 9. Göhner, C. et al. (2015) A New Enzyme-linked Sorbent Assay (ELSA) to Quantify Syncytiotrophoblast Extracellular Vesicles in Biological Fluids.Am J Reprod Immunol. 73 (6): 582-8. 10. Fisken, J. et al. (1989) Serum placental-like alkaline phosphatase (PLAP): a novel combined enzyme linked immunoassay for monitoring ovarian cancer.J Clin Pathol. 42 (1): 40-5. 11. Orozco AF et al. (2009) Placental release of distinct DNA-associated micro-particles into maternal circulation: reflective of gestation time and preeclampsia.Placenta. 30 (10): 891-7. 12. Dankers, P.Y.W. et al. (2015) Convenient formulation and application of a supramolecular ureido-pyrimidinone modified poly(ethylene glycol) carrier for intrarenal growth factor deliveryEuropean Polymer Journal. Jul 2 [Epub ahead of print]
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